X4500
Xanthine Oxidase from bovine milk
Grade III, ammonium sulfate suspension, ≥0.8 units/mg protein
Sinônimo(s):
Schardinger enzyme, XnOx, hypoxanthine oxidase, XOD, Xanthine:oxygen oxidoreductase
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About This Item
Número CAS:
Número da licença da enzima:
1.17.3.2.
Número CE:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54
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fonte biológica
bovine milk
tipo
Grade III
forma
ammonium sulfate suspension
atividade específica
≥0.8 units/mg protein
técnica(s)
inhibition assay: suitable
cor
faint brown to dark brown
nº de adesão UniProt
atividade externa
uricase ≤0.1%
temperatura de armazenamento
2-8°C
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Descrição geral
Research area: Cell Cycle
Xanthine Oxidase (XO) belongs to the class of complex metalloflavoproteins. It is produced by oxidation of sulfhydryl residues or by proteolysis of xanthine dehydrogenase (XDH). XO is characterized with two flavin molecules (FAD), two molybdenum atoms, and eight iron atoms bound per enzymatic unit.
Xanthine Oxidase (XO) belongs to the class of complex metalloflavoproteins. It is produced by oxidation of sulfhydryl residues or by proteolysis of xanthine dehydrogenase (XDH). XO is characterized with two flavin molecules (FAD), two molybdenum atoms, and eight iron atoms bound per enzymatic unit.
Formerly E.C. 1.1.3.22
Aplicação
Xanthine Oxidase from bovine milk has been used:
- as a source for superoxide generation
- for the degradation of hypoxanthine
- for the detection of reactive oxygen species (ROS) in lipid model by electron spin resonance (ESR) spin trap method
- as a constituent of assay mixture in xanthine oxidase inhibition assay
Ações bioquímicas/fisiológicas
Xanthine Oxidase (XO)catalyzes the hydroxylation of hypoxanthine to xanthine and xanthine to uric acid. Xanthine oxidase activity is inhibited by folic acid. [7] and various other inhibitors including 3,4-Dihydroxy-5-nitrobenzaldehyde (DHNB), febuxostat and allopurinol. XO and xanthine dehydrogenase (XDH) plays a vital role in the last two steps in the formation of urate. Elevated levels of XO has been observed in the serum of chronic liver disease patients. Therefore, XO can be used as a biomarker for the detection of liver disease.
Xanthine oxidase activity is inhibited by folic acid.
Xanthine oxidase is a molybdenum-containing enzyme that is found in the cytosol, and may be strongly inhibited by flavonoids. It plays a vital role in the metabolism of some drugs, as well as purines and pyrimidines. It is also known to be a biological source of reactive oxygen species.
Definição da unidade
One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.
forma física
Suspension in 2.3 M (NH4)2SO4, 10 mM sodium phosphate buffer, pH 7.8, containing 1 mM EDTA and 1 mM sodium salicylate
Nota de preparo
Chromatographically purified
Armazenamento e estabilidade
Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized
persons
persons
Nota de análise
Protein determined by biuret
substrato
Nº do produto
Descrição
Preços
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Resp. Sens. 1
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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Serum xanthine oxidase in human liver disease
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The American Journal of Gastroenterology, 96(4), 1194-1199 (2001)
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Artigos
Instructions for working with enzymes supplied as ammonium sulfate suspensions
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