10110434001

Xanthine Oxidase (XOD)

from cow milk

• Sinônimo(s): XOD
• Número da licença da enzima: 1.1.3.22 (BRENDA, IUBMB)
• Código UNSPSC: 12352204

Propriedades

• fonte biológica: bovine milk
• Nível de qualidade: 100
• forma: suspension
• atividade específica: ~1 units/mg protein (At 25 °C with xanthine as the substrate.)
• embalagem: pkg of 1 mL (20 U)
• fabricante/nome comercial: Roche
• pH ideal: 8.5-9.0
• Condições de expedição: wet ice
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

Xanthine Oxidase (XOD) is a metal flavoprotein. It has FAD, molybdenum and iron in the ratio 2:2:8.

Xanthine:oxygen oxidoreductase

Aplicação

Xanthine Oxidase (XOD) has been used in the assessment of XOR-mediated NO production from NDHP and assessment of nitrite-derived NO in liver and purified XOR.

Xanthine Oxidase has been used to study tyrosine nitration.

Ações bioquímicas/fisiológicas

Xanthine Oxidase (XOD) exhibits a broad substrate specificity including aldehydes, purines and pteridines. Furthermore, this enzyme reduces oxygen to generate superoxide, hydrogen peroxide and reactive oxygen species (ROS). It also reduces nitrite to yield reactive nitrogen species (RNS), such as peroxynitrite and nitric oxide. Owing to its ability to generate RNS and ROS, XOD might play an important role as an antimicrobial agent in the neonatal gut, thereby complementing endogenous enzyme of the intestinal epithelium.

Qualidade

Contaminants: <0.005% guanase, NP and uricase, each, <0.05% ADA, <0.05% alkaline phosphatase (4-nitrophenyl phosphate as the substrate)
Note: Chromatographically purified.

Sequência

XOD is a dimer. Each subunit contains 1 atom of molybdenum, 2 iron-sulfur centers (non-heme iron, ferredoxin-type) and 1 molecule of FAD.

Definição da unidade

One unit (U) xanthine oxidase will produce 1 μmol of uric acid (E_293nm = 12.2 mmol ^-1 x L x cm^-1) from the oxidation of 1 μmol of xanthine in 1 min at 25 °C and pH8.5.

forma física

Suspension in 3.2 M ammonium sulfate solution, 10 mM EDTA, pH approximately 8

Nota de preparo

Activator: O₂
Stabilizers: The substance is stabilized by the addition of EDTA; salicylate is not added.

Outras notas

For life science research only. Not for use in diagnostic procedures.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): does not flash
• Ponto de fulgor (°C): does not flash