SRP6169

IL-1β human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Sinônimo(s): Catabolin, Endogenous pyrogen (EP), Leukocyte endogenous mediator (LEM), Lymphocyte-activating factor (LAF), Mononuclear cell factor (MCF)
• Número CAS: 97599-21-8
• Código UNSPSC: 12352202
• NACRES: NA.32

Propriedades

• fonte biológica: human
• recombinante: expressed in HEK 293 cells
• Ensaio: ≥95% (SDS-PAGE)
• Formulário: lyophilized
• peso molecular: (18 and 25 kDa, monomer, glycosylated)
• embalagem: pkg of 10 μg
• técnica(s): cell culture | mammalian: suitable
• nº de adesão NCBI: P01584
• Condições de expedição: dry ice
• temperatura de armazenamento: −70°C
• Informações sobre genes: human ... IL1B(3553)

Descrição

Descrição geral

Interleukin-1β (IL1B) is produced by activated macrophages. It is synthesized as pro-IL-1β which is changed to active form IL-1β with the help of pro-inflammatory protease caspase-1. IL-1β belongs to the IL-1 gene family. It is a proinflammatory cytokine. The gene is mapped to human chromosome 2q14. In comparison with the Escherichia coli expressed protein, IL-1β is 50% more potent in promoting human CD4+ T cell differentiation into Th17 (T helper) cells. This cytokine is produced in a serum free, chemically defined media.

Aplicação

• IL-1β (IL1B) has been used to check the expression of NGAL (neutrophil gelatinase-associated lipocalin) in epithelial cells after stimulation with proinflammatory cytokines.
• It has been used to check anti-inflammatory activities of N-Acetylglucosamine in human chondrocytes.
• It has been used to create inflammatory endothelium in human coronary artery endothelial cells.

Ações bioquímicas/fisiológicas

IL-1β (IL1B) stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation and fibroblast growth factor activity. IL-1β proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. IL-1β is present in chronic inflamed tissues and also in tissues going for fibrogenesis. It brings neutrophil and macrophage to the site of infection. Mutations in IL-1β gene are linked with renal manifestations and renal sequelae in Henoch-Schönlein purpura. It is also associated with destruction of β-cell in type 1 diabetes.

forma física

Lyophilized from a PBS solution.

Nota de preparo

Centrifuge the vial prior to opening.

Reconstituição

Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable