SRP3058

IFN-γ human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• Sinônimo(s): Immune Interferon, MAF, T cell interferon, type II interferon
• Código UNSPSC: 12352202
• NACRES: NA.32

Propriedades

• fonte biológica: human
• recombinante: expressed in E. coli
• Ensaio: ≥98% (HPLC); ≥98% (SDS-PAGE)
• Formulário: lyophilized
• potência: 5.0-10.0 ng/mL ED₅₀
• peso molecular: 16.7 kDa
• embalagem: pkg of 100 μg
• técnica(s): cell culture | mammalian: suitable
• Impurezas: <0.1 EU/μg endotoxin, tested
• cor: white to off-white
• nº de adesão UniProt: P01579
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C
• Informações sobre genes: human ... IFNG(3458)

Descrição

Descrição geral

IFN-γ (interferon γ) is an acid-labile glycosylated interferon produced by CD4 and CD8 T lymphocytes as well as activated NK (natural killer) cells.[1][2][3] IFN-γ receptors are present in most immune cells, which respond to IFN-γ signaling by increasing the surface expression of class I MHC (major histocompatibility complex) proteins. This promotes the presentation of antigen to T-helper (CD4+) cells.[4] The gene is mapped to human chromosome 12q15.[5] Human IFN-γ is species-specific and is biologically active only in human and primate cells. Recombinant human IFN-γ is a 16.7kDa protein containing 143 amino acid residues.

Aplicação

IFN-γ (interferon γ)-human has been used for the generation of inflammatory M1 cells from human monocytic cell line, THP1.[6][7]

Ações bioquímicas/fisiológicas

IFN-γ (interferon γ) signaling in antigen-presenting cells and antigen-recognizing B and T lymphocytes regulate the antigen-specific phases of the immune response.[8][9] It can suppress the generation of anti-inflammatory cytokines and enhance the secretion of proinflammatory cytokines.[10] Additionally, IFN-γ stimulates a number of lymphoid cell functions including the anti-microbial and anti-tumor responses of macrophages, NK (natural killer) cells and neutrophils.[11][12] It is responsible for tumor rejection and is capable of killing tumor cells by autophagy or apoptosis.[3] IFN-γ is also involved in Hepatitis E virus disease pathogenesis.[13]

Sequência

MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ

forma física

Lyophilized from 10 mM Sodium Phosphate, pH 7.4.

Reconstituição

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable