Substrato cromogênico para uso com procedimentos de ELISA com fosfatases ácidas e alcalinas. O substrato gera um produto final solúvel de cor amarela que pode ser lido por espectrofotometria em 405 nm. A reação de pNPP pode ser interrompida com NaOH 3 N e lida em 405 nm.
Phosphatase substrate has been used in the chromogenic detection of adsorbed rabbit IgG to determine coating efficiency.[1]
Outras notas
Substrato para determinação de fosfatases alcalinas e ácidas.
Single step, direct fluorescence immunoassays based on metal enhanced fluorescence (MEF-FIA) applicable as micro plate-, array-, multiplexing-or point of care-format
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