Substrato cromogênico para uso com procedimentos de ELISA com fosfatases ácidas e alcalinas. O substrato gera um produto final solúvel de cor amarela que pode ser lido por espectrofotometria em 405 nm. A reação de pNPP pode ser interrompida com NaOH 3 N e lida em 405 nm.
Outras notas
Substrate for the determination of acid and alkaline phosphatases.
Substrato para determinação de fosfatases alcalinas e ácidas.
Tissue engineering and regenerative medicine, 17(4), 477-493 (2020-05-26)
Insufficient vascularization hampers bone tissue engineering strategies for reconstructing large bone defects. Delivery of prolyl-hydroxylase inhibitors (PHIs) is an interesting approach to upregulate vascular endothelial growth factor (VEGF) by mimicking hypoxic stabilization of hypoxia-inducible factor-1alpha (HIF-1α). This study assessed two
Proceedings of the National Academy of Sciences of the United States of America, 106(8), 2963-2967 (2009-02-04)
It is well known that children need solicitous parenting and a nurturing rearing environment to ensure their normal behavioral development. Early adversity often negatively impacts emotional and mental well-being, but it is less clearly established how much the maturation and
IgA1 complexes containing deglycosylated IgA1, IgG autoantibodies, and a soluble form of the IgA receptor (sCD89), are hallmarks of IgA nephropathy (IgAN). Food antigens, notably gluten, are associated with increased mucosal response and IgAN onset, but their implication in the
Arthritis research & therapy, 16(2), R72-R72 (2014-03-19)
In addition to the pivotal roles of mast cells in allergic diseases, recent data suggest that mast cells play crucial roles in a variety of autoimmune responses. However, their roles in the pathogenesis of autoimmune skeletal muscle diseases have not
Applied microbiology and biotechnology, 83(4), 679-687 (2009-02-19)
Streptomyces coelicolor A3(2) alditol oxidase (AldO) is a soluble monomeric flavoprotein in which the flavin cofactor is covalently linked to the polypeptide chain. AldO displays high reactivity towards different polyols such as xylitol and sorbitol. These characteristics make AldO industrially
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
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