P4762

Papain from papaya latex

lyophilized powder, ≥10 units/mg protein

• Sinônimo(s): Papainase
• Número CAS: 9001-73-4
• Número da licença da enzima: 3.4.22.2 (BRENDA, IUBMB)
• Número CE: 232-627-2
• Número MDL: MFCD00131791
• Código UNSPSC: 12352204
• eCl@ss: 42030311
• NACRES: NA.54

Propriedades

• fonte biológica: papaya (latex)
• Nível de qualidade: 300
• Formulário: lyophilized powder
• atividade específica: ≥10 units/mg protein
• peso molecular: 23.4 kDa
• técnica(s): cell culture | mammalian: suitable; single cell analysis: suitable
• aplicação(ões): diagnostic assay manufacturing
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

Papain is a cysteine protease, found in Carica papaya. It is composed of a single polypeptide chain containing three disulfide bridges and a sulfhydryl group.[1]

Aplicação

Papain from papaya latex has been used:

• to digest carotid artery smooth muscle cells[2]
• in dissecting solutions
• to produce Fab fragments of antibodies[3]
• for cell dissociation since it has been shown to be more effective and less damaging with certain tissues[4][5]
• in the enzyme cocktail for dissociating human fetal retinal tissue[6]
• to digest chondrocytes/scaffold cultures for total DNA quantification for cell proliferation[7]
• as a supplement in neurobasal medium to produce retinal cell suspensions[8]

Suitable for use in preparation of single cell suspension for sequencing.

Used to produce Fab fragments of antibodies. Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues.

Ações bioquímicas/fisiológicas

A cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
pH optimum 6.0-7.0
Also hydrolyzes esters and amides.

Papain with wide specificity,[9] cleaves peptide bonds of basic amino acids, leucine or glycine. It also hydrolyzes esters and amides. Papain is considered to digest protein substrates more broadly than the pancreatic proteases.[10] Papain is commonly used in cell isolation procedures as it is highly efficient and less harmful than other proteases on certain tissues.[11] The use of papain is known to yield more viable smooth muscle cells, retaining the cell′s sensitivity to stimulants.[12] Papain papaya latex has antifungal activity against C. albicans and is a cysteine protease.

• The pH optimum is 6.0-7.0
• Papain hydrolyzes esters and amides

Embalagem

Package size based on protein content

Definição da unidade

One unit will hydrolyze 1.0 micromole of N-alpha-benzoyl-L-arginine ethyl ester (BAEE) per minute at pH 6.2 at 25 deg C.

forma física

Lyophilized powder containing sodium acetate

Nota de preparo

2× Crystallized

Outras notas

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

View more information on papain at www.sigma-aldrich.com/enzymeexplorer.

Informações de segurança

• Pictogramas: GHS08,GHS07
• Palavra indicadora: Danger
• Frases de perigo: H315,H319,H334,H335
• Declarações de precaução: P302 + P352 - P305 + P351 + P338
• Classificações de perigo: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Órgãos-alvo: Respiratory system
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: dust mask type N95 (US), Eyeshields, Faceshields, Gloves