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B5144

Sigma-Aldrich

Bromelain from pineapple stem

≥4 units/mg protein, (chromatography purified)

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100 UNITS
R$ 3.102,00
250 UNITS
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100 UNITS
R$ 3.102,00
250 UNITS
R$ 6.492,00

About This Item

Número CAS:
Número da licença da enzima:
Número CE:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54

R$ 3.102,00


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Formulário

lyophilized powder

Nível de qualidade

atividade específica

(chromatography purified)
≥4 units/mg protein

composição

Protein, 30-50% biuret

solubilidade

H2O: soluble 1.0 mg/mL, clear, colorless

temperatura de armazenamento

−20°C

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Aplicação

Bromelain from pineapple stem has been used as a positive control in sodium dodecyl sulfate (SDS) / polyacrylamide gel electrophoresis (PAGE) zymograms.[1] It has also been used as a Azocasein substrate for the determination of proteolytic activity.[2]
Bromelain may be used to inhibit the biosysnthesis of proinflammatory prostaglandins. It may be used to reduce clotting efficiency. Bromelain, from pineapple stem, has been used to make enzymatic hydrolysates of honeybee-collected pollen [3].

Ações bioquímicas/fisiológicas

Bromelain is a cysteine endopeptidase with broad specificity for cleavage of proteins. Bromelain may be from a stem or piece of fruit. Product P3169 (pGlu-Phe-Leu-p-nitroanilide) may be used as a chromogenic substrate for the assay of bromelain.

Definição da unidade

One unit will release 1.0 μmole of p-nitrophenol from N-α-Z-L-lysine p-nitrophenyl ester per min at pH 4.6 at 25 °C.

forma física

Lyophilized powder containing mannitol and potassium phosphate buffer salts

Nota de preparo

Chromatographically purified
Chromatographically purified. Dissolves in water at 1 mg/mL concentration to form a clear, colorless solution.

Inibidor

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substrato

Nº do produto
Descrição
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Pictogramas

Health hazardExclamation mark

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Órgãos-alvo

Respiratory system

Código de classe de armazenamento

13 - Non Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Influenza A virus envelope contains lipid molecules of the host cell and three integral viral proteins: major hemagglutinin, neuraminidase, and minor M2 protein. Membrane-associated M1 matrix protein is thought to interact with the lipid bilayer and cytoplasmic domains of integral
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Expression of proteases in heterologous hosts remains an ambitious challenge due to severe problems associated with digestion of host proteins. On the other hand, proteases are broadly used in industrial applications and resemble promising drug candidates. Bromelain is an herbal
Azocasein substrate for determination of proteolytic activity: reexamining a traditional method using bromelain samples
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Vaccines against poliomyelitis and influenza contain inactivated forms of poliovirus and influenza virus. These antigens are generated on an industrial scale from the purified active viruses that have been analysed in this study by DSC (differential scanning calorimetry). Multiple unfolding

Questions

  1. Good day! I would like to ask if do you have a Bromelain Powder in CDU? We would like to purchase for our research regarding bromelain enzyme.

    1 answer
    1. Currently, there are no Bromelain products tested with casein and reported in CDU.

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