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P2850

Sigma-Aldrich

Phosphatase Inhibitor Cocktail 1

DMSO solution, mixture of inhibitors

Sinônimo(s):

Phosphatase Inhibitor

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About This Item

Número CE:
200-664-3
Código UNSPSC:
12352200
NACRES:
NA.54
Preço e disponibilidade não estão disponíveis no momento.

Nome do produto

Phosphatase Inhibitor Cocktail 1, DMSO solution

Formulário

DMSO solution

Nível de qualidade

300

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

Descrição geral

Phosphatase Inhibitor Cocktail 1 includes a mixture of inhibitors that will inhibit the L-isozymes of alkaline phosphatase as well as serine/threonine protein phosphatases such as PP1 and PP2A. This product contains microcystinLR, cantharidin, and (−)-p-bromotetramisole. Phosphatase Inhibitor Cocktail 1 has been replaced with P0044.

Aplicação

Phosphatase Inhibitor Cocktail 1 has been used in nuclear protein isolation and electrophoretic mobility shift assay (EMSA) for catenin activation.[1] It has also been used in western blot analysis.[2][3]

Ações bioquímicas/fisiológicas

Phosphatases are active regulators of a number of important biological processes. Inhibition of phosphatases by small molecules is useful to study its biological role.[4]

Quantidade

Recommended usage is a 100-fold dilution.

Nota de análise

Tested on cell extracts from animal tissues such as bovine liver, human placenta, and rabbit muscle, as well as on extracts from A431 and Jurkat cell lines.

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

188.6 °F

Ponto de fulgor (°C)

87 °C

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Certificados de análise (COA)

Lot/Batch Number
0000317618
0000317617
0000317617
0000317618
0000215517

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Artigos

Phosphatase Inhibitor Cocktail

The use of phosphatase inhibitors is critical for all types of phosphorylation studies. Compare phosphatase inhibitor cocktails to select the best one for your research.

Questions

1–6 of 6 Questions  

  1. At what concentration should I use Product P2850, Phosphatase Inhibitor Cocktail 1?

    1 answer

    1. In most circumstannces, the product is used at a 100-fold dilution.  This will inhibit the phosphatases in 5 mg of protein per mL of lysate.

      Helpful?

  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  3. How stable is Product P2850, Phosphatase Inhibitor Cocktail 1?  Can I freeze it?

    1 answer

    1. This reagent, as supplied and stored at 2-8°C, will be stable for four years from the date of manufacturing. Freezing is not recommended, because it may cause precipitation of the inhibitors.

      Helpful?

  4. Is Product P2850, Phosphatase Inhibitor Cocktail 1, suitable for use on yeast?

    1 answer

    1. As far as we know, the phosphatases in mammalian cells also appear in yeast cells. Thus both cocktails, P5726 (Phosphatase cocktail II and P2850 (Phosphatase Inhibitor cocktail I), may be needed with yeast extracts.

      Helpful?

  5. What inhibitors are present in Product P2850, Phosphatase Inhibitor Cocktail 1, and in what concentrations?

    1 answer

    1. Cantharidin (Product Code C7632) inhibits protein phosphatase 2A (PP-2A).  Bromotetramisole (Product Code B8132) inhibits alkaline phosphatases. Microcystin LR (Product Code M2912) inhibits protein phosphatases 1 and 2A (PP-1 and PP-2A). The concentrations of these inhibitors are considered proprietary.

      Helpful?

  6. Product P2850, Phosphatase Inhibitor Cocktail 1, is solid when it arrived. Is it suitable to use?

    1 answer

    1. Since this solution is supplied in DMSO, it may solidify (melting point of DMSO is 16-19 deg C).  Simply allow it to melt before use.  This will not affect the product.

      Helpful?

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