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M5696

Sigma-Aldrich

Myoglobin from equine skeletal muscle

BioUltra, 95-100% (SDS-PAGE)

Sinônimo(s):

Myoglobin from horse skeletal muscle

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About This Item

Número CAS:
Número CE:
Número MDL:
Código UNSPSC:
12352202
NACRES:
NA.61

fonte biológica

equine skeletal muscle

linha de produto

BioUltra

Ensaio

95-100% (SDS-PAGE)

forma

essentially salt-free, lyophilized powder

peso molecular

~17 kDa(lit.)

purificado por

affinity chromatography

Teor de ferro teor

~0.30%

técnica(s)

mass spectrometry (MS): suitable

solubilidade

H2O: soluble 10 mg/mL

nº de adesão UniProt

temperatura de armazenamento

−20°C

Informações sobre genes

horse ... MB(100054434)

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Aplicação

Myoglobin from equine skeletal muscle has been used to induce acute kidney injury (AKI) in mice. It has also been used as a standard for top down mass spectrometry.
Myoglobin is used as a molecular weight marker and a standard for mass spectroscopy and X-ray crystallography.Myoglobin from equine skeletal muscle was used in a study to test experimental protein mixture for validating tandem mass spectral analysis.

Ações bioquímicas/fisiológicas

Myoglobin from horse skeletal muscle is a single chain heme protein containing 153 amino acid residues. It possesses no disulfide bridges or free -SH groups. Myoglobin contains 8 variously sized right-handed helical regions, joined by non-ordered or random coil regions.
Myoglobin is critical to skeletal muscle O2 supply at near-maximum oxygen demand, and prevents anoxia by maintaining PO2 above levels needed to support mitochondrial function.
Myoglobin is critical to skeletal muscle O2 supply at near-maximum oxygen demand, and prevents anoxia by maintaining PO2 above levels needed to support mitochondrial function.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


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S C Powell et al.
Journal of chromatography, 317, 87-92 (1984-12-28)
Urine and serum myoglobin have been separated on an anion-exchange column, packed by the slurry technique. Urine or serum was injected directly into the column and eluted isocratically with Tris buffer. Freshly prepared myoglobin from human muscle gives two peaks
Seok Jong Song et al.
International journal of molecular sciences, 21(22) (2020-11-19)
A recent study showed that early renal tubular injury is ameliorated in Nod-like receptor pyrin domain-containing protein 3 (NLRP3) KO mice with rhabdomyolysis-induced acute kidney injury (RIAKI). However, the precise mechanism has not been determined. Therefore, we investigated the role
Z K Shihabi
Journal of chromatography. B, Biomedical applications, 669(1), 53-58 (1995-07-07)
Capillary electrophoresis was used in this study to separate urinary myoglobin from hemoglobin based on its electrophoretic mobility. Urine was applied directly without any treatment. The separation was accomplished in less than 7 min. Myoglobin extracted from human muscle tissues
Protein Bioinformatics (2017)
Rhabdomyolysis induced AKI via the regulation of endoplasmic reticulum stress and oxidative stress in PTECs
Feng Y, et al.
Royal Society of Chemistry Advances, 6(111), 109639-109648 (2016)

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Chromatograms

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