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M2520

Sigma-Aldrich

Medium 199

HEPES Modification, with Earle′s salts, L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

Sinônimo(s):

M199 Medium, TCM 199

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.75
Preço e disponibilidade não estão disponíveis no momento.

Nível de qualidade

500

Formulário

powder

técnica(s)

cell culture | mammalian: suitable

componentes

L-glutamine: yes
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: no
sodium pyruvate: no
HEPES: 25 mM

Condições de expedição

ambient

temperatura de armazenamento

2-8°C

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Descrição geral

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Aplicação

Medium 199 has been used to:

  • maintain the cumulus-oocyte complex (COC) before maturation and for culturing of these oocytes for in vitro maturation[1]
  • to rinse the aspiration line during the collection of the ovum from German Holstein cows[2]
  • to culture cardiac endothelial cells from rats[3]

Quantidade

Formulated to contain 14.7 grams of powder per liter of medium.

Reconstituição

Supplement with 2.2 g/L sodium bicarbonate.

geralmente comprado junto com este produto

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Preços

suplemento

Nº do produto
Descrição
Preços

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

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Certificados de análise (COA)

Lot/Batch Number
0000401361
0000401336
0000401336
0000277440
0000263114

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In vitro nuclear maturation of bitch oocytes in the presence of polyvinyl-pyrrolidone
Animal Reproduction, 3(1), 70-75 (2018)
Mao Ye et al.
Scientific reports, 4, 4681-4681 (2014-04-16)
The highly specialized endothelial cells in brain capillaries are a key component of the blood-brain barrier, forming a network of tight junctions that almost completely block paracellular transport. In contrast to vascular endothelial cells in other organs, we show that
Jarosław Wieczorek et al.
Animals : an open access journal from MDPI, 10(4) (2020-04-16)
The laparoscopic method of recovering oocytes in goats and sheep is one of the minimally invasive methods used in the biotechnology of animal reproduction. It allows for good quality oocytes that are suitable for in vitro maturation and fertilization to
L González-Fernández et al.
Reproduction, fertility, and development, 30(2), 297-306 (2017-07-07)
Equine cumulus-oocyte complexes (COCs) are classified as compact (cCOC) or expanded (eCOC) and vary in their meiotic competence. This difference could be related to divergent glucose metabolism. To test this hypothesis in the present study, eCOCs, cCOCs and expanded or
A J Korzekwa et al.
Polish journal of veterinary sciences, 19(3), 609-617 (2016-10-21)
Adenomyosis is defined as the presence of glandular foci external to the endometrium of the uterus, either in the myometrium or/and perimetrium, depending on the progress of this dysfunction. To date, we showed that steroids secretion and prolactin expression and
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Artigos

Medium 199 - M199 Formulations

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

Calcium in Cell Culture

Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.

Protocolos

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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