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M5017

Sigma-Aldrich

Medium 199

With Earle′s salts and ʟ-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Sinônimo(s):

Cell Culture Medium, M199 Medium, Medium 199 Solution, TCM 199

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.71

product name

Medium 199, With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

forma

powder

técnica(s)

cell culture | mammalian: suitable

componentes

L-glutamine: yes
phenol red: yes
NaHCO3: no
sodium pyruvate: no
Earle’s salts (5% CO2): yes
HEPES: no

Condições de expedição

ambient

temperatura de armazenamento

2-8°C

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Descrição geral

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Quantidade

Formulated to contain 9.5 grams of powder per liter of medium.

Reconstituição

Supplement with 2.2 g/L sodium bicarbonate.

Código de classe de armazenamento

13 - Non Combustible Solids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Artigos

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

Protocolos

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

Conteúdo relacionado

Discover the formulation for Medium 199 for cell culture applications, including vaccine development.

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