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M4530

Sigma-Aldrich

Medium 199

With Earle′s salts, ʟ-glutamine and sodium bicarbonate, sterile liquid, suitable for cell culture

Sinônimo(s):

M199 Medium, TCM 199

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.75

product name

Medium 199, With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

esterilidade

sterile-filtered

forma

liquid

técnica(s)

cell culture | mammalian: suitable

Impurezas

endotoxin, tested

componentes

NaHCO3: yes
L-glutamine: yes
HEPES: no
Earle’s salts (5% CO2): yes
phenol red: yes
sodium pyruvate: no

Condições de expedição

ambient

temperatura de armazenamento

2-8°C

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Descrição geral

Complete liquid version of the original M 199 formulation published by Morgan.
Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Aplicação

Medium 199 has been used:
  • as a suspension media to maintain the primary neuronal cultures
  • as a component of the collagen gel for ex vivo embryonic heart culture
  • to maintain the ovarian tissue sections as part of the slow-freezing protocol

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Artigos

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.

Conteúdo relacionado

Discover the formulation for Medium 199 for cell culture applications, including vaccine development.

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