Pular para o conteúdo
Merck
Todas as fotos(1)

Documentos

L9379

Sigma-Aldrich

Lectin from Phytolacca americana (pokeweed)

lyophilized powder

Sinônimo(s):

PWM, Pokeweed mitogen

Faça loginpara ver os preços organizacionais e de contrato
Todas as fotos(1)

About This Item

Número MDL:
MFCD00161973
Código UNSPSC:
12352202
NACRES:
NA.32

esterilidade

aseptically filled

Nível de qualidade

200

forma

lyophilized powder

potência

≤0.3 μg/mL mitogenic activity

temperatura de armazenamento

2-8°C

Procurando produtos similares? Visita Guia de comparação de produtos

Categorias relacionadas

Descrição geral

Lectins are carbohydrate-binding proteins, omnipresent, found in fungi, plants and animals. The structure of lectin is diversely studied in plants and animals. The secondary structure of this protein is rich in β-strands and possesses a carbohydrate binding sites on the surface.

Aplicação

Lectin from Phytolacca americana (pokeweed) has been used:
  • as a medium supplement for mononuclear cell incubation, to induce lymphocyte proliferation
  • as a positive control in the study of the role of chemokine (C–C motif) ligand 2 (CCL2) in proliferation and apoptosis of decidual leukocytes
  • as a supplement in the RPMI (Roswell park memorial institute) 1640 medium for peripheral blood lymphocyte cultures

Ações bioquímicas/fisiológicas

Lectin is known to be useful in glycoconjugate characterizing, imaging and targeting. Its use in microarray assay, enable efficient glycome profiling. This is because of its specific interaction with oligosaccharides, glycoproteins and glycolipids. In plants and fungi, lectin defends against pathogens/feeders. Lectin participates in host recognition and tissue adhesion, thereby aids in the pathogenesis of microorganism.
PWM is extracted from pokeweed roots. This protein has hemagglutinating, leukoagglutinating, and mitogenic properties. PWM is not blood group specific, but has affinity for N-acetyl-β-D-glucosamine oligomers.

forma física

Partially purified TCA precipitate.
Essentially salt-free.

Nota de análise

Mitogenic activity was determined by BrdU incorporation in HeLa cell cultures.

Pictogramas

Health hazard
GHS08

Palavra indicadora

Danger

Frases de perigo

H317,H334

Classificações de perigo

Resp. Sens. 1 - Skin Sens. 1

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Assessment of cellular immune responses of healthy and diseased Tasmanian devils (Sarcophilus harrisii)
Kreiss A, et al.
Developmental and Comparative Immunology, 32(5), 544-553 (2008)
The use of a novel combination of diagnostic molecular and cytogenetic approaches in horses with sexual karyotype abnormalities: a rare case with an abnormal cellular chimerism
Demyda P, et al.
Theriogenology, 81(8), 1116-1122 (2014)
B Hejdeman et al.
HIV medicine, 4(2), 101-110 (2003-04-19)
To study the impact of effective highly active antiretroviral therapy (HAART) on the preservation of long-term CD4 memory cells induced by vaccines in HIV-1-infected patients. Thirty HIV-1-positive patients on HAART with undetectable viral load were randomized into three groups: 10
Nishaki Mehta et al.
Vaccine, 28(21), 3672-3678 (2010-04-02)
Hepatitis B-specific memory B cell (HSMBC) frequencies were measured following hepatitis B vaccination in 15 HIV uninfected and 12 HIV infected adolescents. HSMBC were detected at significantly lower frequencies in HIV infected than in HIV uninfected individuals. The detection of
W E Müller et al.
European journal of biochemistry, 183(2), 391-396 (1989-08-01)
Previously it was established [Pahwa, S., Pahwa, R., Saxinger, C., Gallo, R. C. & Good, R. A. (1985) Proc. Natl. Acad. Sci. USA 82, 8198] that nonviable preparations of human immunodeficiency virus 1 (HIV-1) abolish the proliferative response of human
Exibir todos os documentos relacionados

Artigos

Lectin Selection Guide: A Useful Guide for Selecting the Right Lectin

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica