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G9284

Sigma-Aldrich

Guanidine hydrochloride solution

Colorless liquid, 7.8 - 8.3 M, pH- 4.5 - 5.5

Sinônimo(s):

Aminoformamidine hydrochloride, Guanidinium chloride, Guanidium chloride

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About This Item

Fórmula empírica (Notação de Hill):
CH5N3 · HCl
Número CAS:
50-01-1
Peso molecular:
95.53
Beilstein:
3591990
Número MDL:
MFCD00013026
Código UNSPSC:
12352107
ID de substância PubChem:
24895369
NACRES:
NA.55

forma

liquid

densidade

1.18 g/mL at 25 °C (lit.)

cadeia de caracteres SMILES

Cl.NC(N)=N

InChI

1S/CH5N3.ClH/c2-1(3)4;/h(H5,2,3,4);1H

chave InChI

PJJJBBJSCAKJQF-UHFFFAOYSA-N

Informações sobre genes

human ... KCNA1(3736) , KCNA10(3744) , KCNA2(3737) , KCNA3(3738) , KCNA4(3739) , KCNA5(3741) , KCNA6(3742) , KCNA7(3743) , KCNB1(3745) , KCNB2(9312) , KCNC1(3746) , KCNC2(3747) , KCNC3(3748) , KCNC4(3749) , KCND1(3750) , KCND2(3751) , KCND3(3752) , KCNF1(3754) , KCNG1(3755) , KCNG2(26251) , KCNG3(170850) , KCNG4(93107) , KCNH1(3756) , KCNH2(3757) , KCNH3(23416) , KCNH4(23415) , KCNH5(27133) , KCNH6(81033) , KCNH7(90134) , KCNH8(131096) , KCNQ1(3784) , KCNQ2(3785) , KCNQ3(3786) , KCNQ4(9132) , KCNQ5(56479) , KCNS1(3787) , KCNS2(3788) , KCNS3(3790) , KCNV1(27012) , KCNV2(169522)

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Aplicação

Guanidine hydrochloride solution has been used:
  • for protein denaturation
  • to homogenize hypothalamus, thalamus, prefrontal cortex, and brainstem samples for total β-amyloid (Aβ) extraction and Aβ42 quantification
  • as a chaotropic denaturant to treat human immunodeficiency virus-1 (HIV-1) samples to study its effects on HIV-1 sensitivity and to assay protein stability

Ações bioquímicas/fisiológicas

Guanidine‐HCl (Gnd-HCl), an efficient chaotropic agent, is broadly used in RNA isolation, globular protein denaturation, and protein refolding studies as a denaturant. It is an appropriate sample buffer for the quick and effective elution of affinity-purified protein complexes and the subsequent protein digestion step. GnHCl can be used to extract proteins in extracellular matrix (ECM)‐rich tissues including bone, cartilage, ligament, and tendon. Gnd-HCl is not used in proteomics experiments due to its interference with trypsin activity at low molar concentrations and tendency to precipitate when combined with sodium dodecyl sulfate (SDS). It is appropriate for performing rapid and single-step affinity-purification mass spectrometry (AP-MS) experiments.

Pictogramas

Exclamation mark
GHS07

Palavra indicadora

Warning

Frases de perigo

H302 + H332,H315,H319

Classificações de perigo

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Sigma-Aldrich

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Tiocianato de guanidina

Jianwen Xu et al.
Journal of pharmaceutical sciences, 110(12), 3819-3828 (2021-09-11)
The purpose of this investigation was to highlight the utility of nuclear magnetic resonance (NMR) as a multi-attribute method for the characterization of therapeutic antibodies. In this case study, we compared results from isothermal chemical denaturation (ICD) and NMR with
Nitish Agrawal et al.
PloS one, 6(6), e21339-e21339 (2011-07-09)
The HIV-1 envelope glycoprotein (Env) spike is challenging to study at the molecular level, due in part to its genetic variability, structural heterogeneity and lability. However, the extent of lability in Env function, particularly for primary isolates across clades, has
Jessica Colby-Milley et al.
PloS one, 10(6), e0130177-e0130177 (2015-06-16)
In addition to cognitive decline, individuals affected by Alzheimer's disease (AD) can experience important neuropsychiatric symptoms including sleep disturbances. We characterized the sleep-wake cycle in the TgCRND8 mouse model of AD, which overexpresses a mutant human form of amyloid precursor
Yalda Ashraf Kharaz et al.
Proteomics, 17(13-14) (2017-05-27)
Exploring the tendon proteome is a challenging but important task for understanding the mechanisms of physiological/pathological processes during ageing and disease and for the development of new treatments. Several extraction methods have been utilised for tendon mass spectrometry, however different
Jon W Poulsen et al.
Journal of proteome research, 12(2), 1020-1030 (2012-11-29)
Protein digestion is an integral part of the "shotgun" proteomics approach and commonly requires overnight incubation prior to mass spectrometry analysis. Quadruplicate "shotgun" proteomic analysis of whole yeast lysate demonstrated that Guanidine-Hydrochloride (Gnd-HCl) protein digestion can be optimally completed within
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