E5389

Erythromycin

powder, suitable for cell culture, BioReagent

• Sinônimo(s): E-Mycin, Erythrocin
• Fórmula empírica (Notação de Hill): C₃₇H₆₇NO₁₃
• Número CAS: 114-07-8
• Peso molecular: 733.93
• Beilstein: 75279
• Número CE: 204-040-1
• Número MDL: MFCD00084654
• Código UNSPSC: 12352207
• ID de substância PubChem: 24894612
• NACRES: NA.76

Propriedades

• product name: Erythromycin, BioReagent, suitable for cell culture
• linha de produto: BioReagent
• forma: powder
• potência: ≥850 μg per mg
• técnica(s): cell culture | mammalian: suitable
• Impurezas: ≤0.1 EU/mg endotoxin
• cor: white
• pf: 133 °C
• solubilidade: 2 M HCl: 50 mg/mL (Stock solutions should be stored at 2-8 °C. Stable at 37 °C for 3 days.); ethanol: soluble (Stock solutions should be stored at 2-8 °C. Stable at 37 °C for 3 days.)
• espectro de atividade do antibiótico: Gram-negative bacteria; Gram-positive bacteria
• Modo de ação: protein synthesis | interferes
• cadeia de caracteres SMILES: CC[C@H]1OC(=O)[C@H](C)[C@@H](O[C@H]2C[C@@](C)(OC)[C@@H](O)[C@H](C)O2)[C@H](C)[C@@H](O[C@@H]3O[C@H](C)C[C@@H]([C@H]3O)N(C)C)[C@](C)(O)C[C@@H](C)C(=O)[C@H](C)[C@@H](O)[C@]1(C)O
• InChI: 1S/C37H67NO13/c1-14-25-37(10,45)30(41)20(4)27(39)18(2)16-35(8,44)32(51-34-28(40)24(38(11)12)15-19(3)47-34)21(5)29(22(6)33(43)49-25)50-26-17-36(9,46-13)31(42)23(7)48-26/h18-26,28-32,34,40-42,44-45H,14-17H2,1-13H3/t18-,19-,20+,21+,22-,23+,24+,25-,26+,28-,29+,30-,31+,32-,34+,35-,36-,37-/m1/s1
• chave InChI: ULGZDMOVFRHVEP-RWJQBGPGSA-N
• Informações sobre genes: human ... ABCB1(5243) , CYP3A4(1576) , MLNR(2862) ; mouse ... Abcb1a(18671) , Abcb1b(18669)

Descrição

Descrição geral

Chemical structure: macrolide

Erythromycin is an antibiotic produced by growth of certain strains of Streptomyces erythreus. This product is composed largely of erythromycin A with small amounts of erythromycins B and C and is recommended for concentration at 100 mg/L. Concentrations between 50 and 200 mg/L have also proven effective in controlling bacterial growth. Erythromycin has been used as a motilin receptor agonist, to block respiratory glycoconjugate secretion in human airways in vitro, and for selecting plasmid-cured and recombinant lactococcus lactis MG1363 strains.

Aplicação

Erythromycin has been used:

• as a supplement for nutrient broth medium for culturing green fluorescent protein (GFP)- expressing E. coli
• as a model drug to determine small intestinal (SMI) microtissue viability using the MTT assay{254
• as an antibiotic to study the treatment strategies of chronic infections

Ações bioquímicas/fisiológicas

Mode of Action: Erythromycin acts by inhibiting elongation at the transpeptidation step, specifically aminoacyl translocation from the A-site to P-site by binding to the 50s subunit of the bacterial 70s rRNA complex.

Antimicrobial Spectrum: This product acts against both gram-negative and gram-positive bacteria.

Atenção

This product is stable in solution at 37°C for 3 days. Stock solutions should be stored at 2-8°C.

Nota de preparo

This product is soluble in water at 2 mg/mL, with a 0.067% solution in water yielding a pH of 8.0-10.5. It is also soluble in ethanol at 50 mg/mL, yielding a clear, colorless to faint yellow solution. It is freely soluble in alcohol, acetone, chloroform, acetonitrile and ethyl acetate but forms salts with acids. All solutions should be protected from light.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)