D8062

DMEM/F-12

With ʟ-glutamine, sodium bicarbonate and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

• Sinônimo(s): Mistura 1:1 de DME/F-12
• Número MDL: MFCD00217417
• Código UNSPSC: 12352207
• NACRES: NA.75

Propriedades

• Nome do produto: Meio de Eagle modificado por Dulbecco/Mistura de nutrientes de Ham F-12, With L-glutamine and sodium bicarbonate, without HEPES, liquid, sterile-filtered, suitable for cell culture
• Nível de qualidade: 400
• esterilidade: sterile-filtered
• Formulário: liquid
• técnica(s): cell culture | mammalian: suitable
• Impurezas: endotoxin, tested
• componentes: glucose: 3.15 g/L; NaHCO₃: yes; L-glutamine: yes; phenol red: yes; HEPES: no; sodium pyruvate: 0.055 g/L
• Condições de expedição: ambient
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

Media are supplemented with a defined mixture of nutrients, growth factors, and hormones instead of serum. A 1:1 mixture of Dulbecco′s Modified Eagle′s medium (DME) and Ham′s F-12 nutrient mixture is commonly used for these defined studies. To compensate for the loss of buffering capacity caused by eliminating serum, a final concentration of 15 mM HEPES buffer can be added to the formulations. It allows the investigator to optimize the buffering system.

Aplicação

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used:

• to maintain human lung and colorectal cancer cell lines for the biological evaluation of the secondary metabolites[1]
• to culture human kidney-2 (HK-2) cells and as a component of the assay medium for lysophosphatidylcholine (LPC) treatment[2]
• to culture mouse squamous cell carcinoma (SCCVII) tumor cells[3]

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable