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D8437

Sigma-Aldrich

DMEM/F-12

With ʟ-glutamine, HEPES, sodium pyruvate and sodium bicarbonate, sterile liquid, suitable for cell culture

Sinônimo(s):

Mistura 1:1 de DME/F-12

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About This Item

Número MDL:
MFCD00217417
Código UNSPSC:
12352207
NACRES:
NA.75

product name

Meio de Eagle modificado por Dulbecco/Mistura de nutrientes de Ham F-12, With L-glutamine, 15 mM HEPES, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

esterilidade

sterile-filtered

forma

liquid

técnica(s)

cell culture | mammalian: suitable

Impurezas

endotoxin, tested

componentes

glucose: 3.15 g/L
HEPES: yes
L-glutamine: yes
phenol red: yes
sodium pyruvate: 0.055 g/L

Condições de expedição

ambient

temperatura de armazenamento

2-8°C

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Descrição geral

Dulbecco′s Modified Eagle′s medium (DMEM), a regularly used cell culture medium, is a source of energy-rich media that includes glucose, vitamins, and amino acids. A 1:1 mixture of Dulbecco′s Modified Eagle′s medium (DME) and Ham′s F-12 nutrient mixture is commonly used in cell culture research. To compensate for the loss of buffering capacity caused by removing serum, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer may be added in the formulations at a final concentration of 15 mM.

Aplicação

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham:
  • has been used as a component in the culture medium to culture primary culture-activated human hepatic stellate cells
  • has been used to culture the cartilage biopsies
  • may be used for the propagation of Cowdria ruminantium

Outras notas

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

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