D5319
Deoxyribonuclease I bovine
recombinant, expressed in Pichia pastoris, buffered aqueous glycerol solution, ≥5,000 units/mg protein
Sinônimo(s):
DNAse I, Deoxyribonucleate 5′-oligonucleotido-hydrolase
Faça loginpara ver os preços organizacionais e de contrato
Selecione um tamanho
500 μG
R$ 2.182,00
2 MG
R$ 7.295,00
R$ 2.182,00
Previsão de entrega em01 de junho de 2025
Selecione um tamanho
Alterar visualização
500 μG
R$ 2.182,00
2 MG
R$ 7.295,00
About This Item
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54
R$ 2.182,00
Previsão de entrega em01 de junho de 2025
Produtos recomendados
fonte biológica
bovine
Nível de qualidade
recombinante
expressed in Pichia pastoris
Ensaio
≥95%
Formulário
buffered aqueous glycerol solution
atividade específica
≥5,000 units/mg protein
peso molecular
~39 kDa
técnica(s)
DNA extraction: suitable
adequação
suitable for molecular biology
aplicação(ões)
diagnostic assay manufacturing
atividade externa
RNAse and protease, free
Condições de expedição
dry ice
temperatura de armazenamento
−20°C
Procurando produtos similares? Visita Guia de comparação de produtos
Categorias relacionadas
Aplicação
DNAse I from Sigma was used to treat nuclear lysate to obtain single nucleosomes in a study.[1] The enzyme has also been for the preparation and harvest of mice mammary glands.[2]
Deoxyribonuclease I bovine has been used in a study to compare the initial actions of spleen deoxyribonuclease and pancreatic deoxyribonuclease. Deoxyribonuclease I bovine has also been used in a study to investigate deoxythymidine 3′, 5′-di-p-nitrophenyl phosphate as a synthetic substrate for bovine pancreatic deoxyribonuclease.
Used for the removal of DNA from protein samples.
Ações bioquímicas/fisiológicas
DNase I is an endonuclease that acts on phosphodiester bonds (adjacent to pyrimidines) to produce polynucleotides with terminal 5′-phosphates. The pH optimum is found to be between 7 and 8. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion.[3] 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS)[4] and actin[5] are known to inhibit the enzyme activity.
Digests single- and double-stranded DNA to a mixture of mono- and oligonucleotides carrying 5′ phosphates and 3′ OH termini. This catalytic activity is divalent ion-dependent. In the presence of Mg2+, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn2+, both strands can be cleaved.
Características e benefícios
- RNA purification by removing DNA
- Prepare DNA for nick translation1
- Footprinting assays to determine DNA-protein interactions2
Definição da unidade
One unit will produce a ΔA260 of 0.001 per min per mL reaction mixture using calf thymus DNA at pH 5.0 and 25°C
forma física
Supplied as a solution in 4 mg/ml glycine pH 5.0, 5 mM calcium acetate and 50% glycerol
Nota de preparo
Produced without using any animal cells or animal derived materials.
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Escolha uma das versões mais recentes:
Certificados de análise (COA)
Lot/Batch Number
Não está vendo a versão correta?
Se precisar de uma versão específica, você pode procurar um certificado específico pelo número do lote ou da remessa.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Os clientes também visualizaram
Wendy A Kellner et al.
Nucleic acids research, 41(20), 9274-9283 (2013-08-16)
Brd4 is a double bromodomain protein that has been shown to interact with acetylated histones to regulate transcription by recruiting Positive Transcription Elongation Factor b to the promoter region. Brd4 is also involved in gene bookmarking during mitosis and is
Hongyuan Xing et al.
Journal of nanobiotechnology, 19(1), 264-264 (2021-09-08)
Exosome therapy is a promising therapeutic approach for intervertebral disc degeneration (IVDD) and achieves its therapeutic effects by regulating metabolic disorders, the microenvironment and cell homeostasis with the sustained release of microRNAs, proteins, and transcription factors. However, the rapid clearance
Alice S Kaanta et al.
Breast cancer research : BCR, 15(4), R65-R65 (2013-08-21)
The mouse mammary gland provides a powerful model system for studying processes involved in epithelial tissue development. Although markers that enrich for mammary stem cells and progenitors have been identified, our understanding of the mammary developmental hierarchy remains incomplete. We
Heidi Hempel Sullivan et al.
The Journal of pathology, 253(4), 415-426 (2020-12-19)
We reported previously that high numbers of mast cells in benign (extra-tumoral) regions of the prostate are associated with worse outcomes after radical prostatectomy including biochemical recurrence and the development of metastases. Herein, with a cohort of 384 men, we
Properties of chromatographically purified bovine pancreatic deoxyribonuclease.
P A Price et al.
The Journal of biological chemistry, 244(3), 917-923 (1969-02-10)
Active Filters
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica
