A7080
Amphiregulin human
≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Sinônimo(s):
AREG, CRDGF, SDGF, AR
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0.1 MG
R$ 6.252,00
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0.1 MG
R$ 6.252,00
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R$ 6.252,00
Check Cart for Availability
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Nome do produto
Amphiregulin human, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture, ≥97% (SDS-PAGE)
fonte biológica
human
Nível de qualidade
recombinante
expressed in E. coli
Ensaio
≥97% (SDS-PAGE)
Formulário
lyophilized powder
potência
10-100 ng/mL ED50/EC50
peso molecular
~11 kDa
embalagem
pkg of 1 mg
condição de armazenamento
avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
técnica(s)
cell culture | mammalian: suitable
Impurezas
endotoxin, tested
nº de adesão UniProt
temperatura de armazenamento
−20°C
Informações sobre genes
human ... AREG(374)
Ações bioquímicas/fisiológicas
Also known as keratinocyte autocrine factor (KAF); expression of amphiregulin mRNA has been found in numerous carcinoma cell lines and epithelial cells of several human tissues including colon, stomach, breast, ovary, and kidney.
Componentes
Human amphiregulin is produced by a DNA sequence that encodes a 98 amino acid residue form of mature human amphiregulin. This corresponds to amino acid residues 101-198 in E. coli. Amphiregulin is a glycoprotein produced in response to treatment of the cell line MCF-7, a human breast carcinoma cell line, with PMA, a protein kinase C activator and potent tumor promoter.
Atenção
This product should be stored at -20°C. After preparation, the product can be stored at 2-8°C for one month, or frozen in aliquots at -20°C or -70°C for more extended storage.
forma física
Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4 with 50 μg BSA per 1 μg as a carrier protein.
Nota de preparo
It should be reconstituted by adding sterile PBS containing 0.1% HAS or BSA to a concentration greater than 10 μg/mL.
Nota de análise
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Marquardt, H. et al. (1984) Science 223:1079. The ED50 for this effect is typically 5-15 ng/mL.
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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