A5977
Anti-VSV-G−Peroxidase antibody, Mouse monoclonal
1.0-1.5 mg/mL, clone P5D4, purified from hybridoma cell culture
Sinônimo(s):
Monoclonal Anti-VSV Glycoprotein
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About This Item
Código UNSPSC:
12352203
NACRES:
NA.56
R$ 2.928,00
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Produtos recomendados
fonte biológica
mouse
Nível de qualidade
conjugado
peroxidase conjugate
forma do anticorpo
purified from hybridoma cell culture
tipo de produto de anticorpo
primary antibodies
clone
P5D4, monoclonal
Formulário
lyophilized powder
concentração
1.0-1.5 mg/mL
técnica(s)
western blot: 1:1,000 using 20-50 ng of a purified VSV-G tagged fusion protein
Isotipo
IgG1
Condições de expedição
wet ice
temperatura de armazenamento
2-8°C
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Descrição geral
Monoclonal Anti-VSV-G-Peroxidase is a lyophilized preparation of the purified immunoglobulin fraction of monoclonal Anti-VSV-G (mouse IgG1 isotype) isolated from ascites fluid of the P5D4 hybridoma, conjugated to horseradish peroxidase (HRP). The antibody is derived from the P5D4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with a synthetic peptide. Vesicular Stomatitis Virus Glycoprotein (VSV-G) exists as a trimer and comprises of four domains, among which domain III is a pleckstrin homology (PH) domain.[1]
Especificidade
The antibody recognizes an epitope containing the five carboxy-terminal amino acids of VSV Glycoprotein. In infected cells, the antibody localizes the immature forms of VSV-G in the rough endoplasmic reticulum (RER) and in the cisternae of Golgi complex, as well as mature VSV-G at the cell surface and in the budding virus. The antibody does not stain the secreted form of VSV-G which lacks the membrane and the cytoplasmic domain. This antibody has been used for studies on the role of the cytoplasmic domain on newly-synthesized VSV-G during transfer to the plasma membrane and cell surface, using micro-injected antibody, immunoblotting, immunoprecipitation, immunocytochemistry and immunoelectron microscopy. The antibody has been used for the detection, immunoprecipitation and immunocytochemical staining of exogenously introduced constructs tagged with the carboxyl-terminus of VSV-G. This tag does not interfere with the function of the studied protein and can be specifically recognized by the P5D4 antibody without cross-reaction with any endogenous protein.
VSV-G tag sequence (YTDIEMNRLGK) on VSV-G tagged fusion proteins when expressed N- or C-terminal to the fusion protein. Recognizes native and denatured forms of VSV-G tagged proteins.
Imunogênio
synthetic peptide containing the 15 carboxy-terminal amino acids (497-511) of Vesicular Stomatitis Virus Glycoprotein (VSV-G), conjugated to KLH.
Aplicação
Monoclonal Anti-VSV-G−Peroxidase antibody produced in mouse may be used immunoblotting.[2]
Ações bioquímicas/fisiológicas
Vesicular Stomatitis Virus Glycoprotein (VSV-G) amino acid sequence YTDIEMNRLGK, corresponding to amino acids 501-511 has been widely used as an epitope tag in expression vectors, enabling the expression of proteins as VSV-G tagged fusion proteins.[2] It constitutes an attractive model to study maturation and intracellular transport of membrane proteins.[1] VSV-G mediates attachment of VSV to the cell surface and induces pH-dependent fusion between viral and target membranes.[1] Besides, its cytoplasmic domain contains information for several intracellular sorting steps, which include efficient export from the endoplasmic reticulum, basolateral delivery and endocytosis.[1].VSV-G is transported through stacks in the Golgi cisternae.[3]
forma física
Supplied as a lyophilized powder. After reconstitution,the solution contains 1% BSA and 0.05% MIT in 0.01 M sodium phosphate buffered saline, pH 7.4.
Reconstituição
Reconstitution with 0.5 mL water results in a solution of 0.01 M sodium phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% MIT.
Armazenamento e estabilidade
Stored the lyophilized product at 2-8 °C. After reconstitution, for extended storage, freeze in working aliquots at –20 °C. For continuous use, the solution may be store at 2-8 °C for up to 1 month. Working dilutions should be discarded. Avoid repeated freeze-thaw.
Exoneração de responsabilidade
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Classificações de perigo
Skin Sens. 1
Código de classe de armazenamento
13 - Non Combustible Solids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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H R Pelham
The Journal of cell biology, 155(7), 1099-1101 (2002-01-05)
The role of vesicles in cargo transport through the Golgi apparatus has been controversial. Large forms of cargo such as protein aggregates are thought to progress through the Golgi stack by a process of cisternal maturation, balanced by a return
Xiangjie Sun et al.
Future virology, 5(1), 85-96 (2010-01-01)
Members of the Rhabdoviridae infect a wide variety of animals and plants, and are the causative agents of many important diseases. Rhabdoviruses enter host cells following internalization into endosomes, with the glycoprotein (G protein) mediating both receptor binding to host
Mary Germino et al.
Yeast (Chichester, England), 23(10), 763-769 (2006-07-25)
Multimeric protein complexes play diverse and vital roles in the cell, but following the composition of these complexes under varying growth conditions can be challenging. Toward that goal, we have designed a vector that permits the double epitope tagging of
H J Stewart et al.
Gene therapy, 16(6), 805-814 (2009-03-06)
Large-scale production of gene therapeutics comprising equine infectious anaemia virus (EIAV) -based lentiviral vectors (LVs) would benefit from the development of producer cell lines enabling the generation of larger quantities of vector than achievable by transient systems. Such cell lines
Yoann G Santin et al.
Current biology : CB, 29(21), 3707-3713 (2019-10-22)
The type VI secretion system (T6SS) is a multiprotein apparatus that injects protein effectors into target cells, hence playing a critical role in pathogenesis and in microbial communities [1-4]. The T6SS belongs to the broad family of contractile injection systems
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