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A1009

Sigma-Aldrich

Albumin methylated from bovine serum

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About This Item

Número MDL:
MFCD00145745
Código UNSPSC:
12352202
eCl@ss:
42010201
NACRES:
NA.26

fonte biológica

bovine

Nível de qualidade

200

forma

powder

origem

USA origin

técnica(s)

affinity chromatography: suitable
fractionation: suitable

Impurezas

≤10% water (Karl Fischer)

nº de adesão UniProt

P02769

temperatura de armazenamento

2-8°C

Informações sobre genes

bovine ... ALB(280717)

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Aplicação

Albumin methylated from bovine serum (mBSA) has been used as a coating for immunoplates in enzyme-linked immunosorbent assay (ELISA) of anti-dsDNA IgG antibodies. It has also been used to induce arthritis in mice models.
As an affinity ligand on diatomaceous earth support (MAK chromatography), a medium for fractionation of different forms of DNA and for separating aminoacyl-tRNAs. Different modes of binding of double-stranded, denatured, and replicating forms of DNA account for the efficacy of the method.

Ações bioquímicas/fisiológicas

Albumin is a major circulating component of the plasma and is a highly soluble multifunctional protein. It plays a role in several physiological and pharmacological processes. Albumin binds to metals, cholesterol, hormones, bile pigments, drugs, and fatty acids to facilitate their transport. It regulates osmotic pressure and distributes fluids between various compartments.

Nota de preparo

Methyl ester of albumin prepared per Mandell, J.D. and Hershey, A.D., Anal. Biochem., 1, 66 (1960).

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificados de análise (COA)

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Albumina sérica bovina lyophilized powder, ≥96% (agarose gel electrophoresis)

Sigma-Aldrich

A2153

Albumina sérica bovina

Kaoru Yamagata et al.
Clinical and experimental rheumatology, 38(4), 670-679 (2019-11-07)
Although articular cartilage contributes to smooth joint motion, once damaged this functionality cannot be recovered. Activation of the IL-6/STAT3 signalling pathway contributes to chondrogenic differentiation of mesenchymal stem cells (MSCs), indicating a role for soluble IL-6R (sIL-6R) during chondrogenesis in
Fractionation of amino acyl-acceptor RNA on a methylated albumin column.
N SUEOKA et al.
Proceedings of the National Academy of Sciences of the United States of America, 48, 1454-1461 (1962-08-01)
Wim B van den Berg et al.
Methods in molecular medicine, 136, 243-253 (2007-11-07)
Antigen induced arthritis is a unilateral T-cell driven model caused by direct injection of an antigen into the knee joint of a FCA preimmunized animal. The chronicity is determined by antigen retention in avascular structures of the joint through charge
T Hofstaetter et al.
Biochimica et biophysica acta, 518(1), 53-60 (1978-03-29)
The binding of methylated albumin to DNA, the basis of the chromatography on columns of kieselguhr coated with methylated albumin (MAK chromatography), was investigated. Scatchard plots revealed only one mode of interaction with fully double-stranded DNA. The complexes should be
Fractionation of nucleic acids with the methylated albumin column.
N SUEOKA et al.
Journal of molecular biology, 4, 161-172 (1962-03-01)
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