I accidentally bought this thinking it was MTT salt to use in a cell proliferation assay but it is MTT Formazan, the water insoluble end product! Therefore, it is useless for the assay. I suppose you could use it as a control to optimize your buffer for solubilizing the insoluble Formazan. The description for this product is misleading and should clarify that this is not what you need for an MTT assay, and refer the customer to MTT salt. That said, this is a nice purple crystalline powder and insoluble in water consistent with MTT Formazan, if that is what you actually want.
88417
MTT Formazan
MTT Formazan
MTT reduction end product, powder
Sinônimo(s):
1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan, Thiazolyl blue formazan
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250 MG
R$ 512,00
1 G
R$ 1.183,00
R$ 512,00
Disponível para enviar em07 de abril de 2025Detalhes
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250 MG
R$ 512,00
1 G
R$ 1.183,00
About This Item
Fórmula empírica (Notação de Hill):
C18H17N5S
Número CAS:
Peso molecular:
335.43
Número MDL:
Código UNSPSC:
12352204
ID de substância PubChem:
NACRES:
NA.83
R$ 512,00
Disponível para enviar em07 de abril de 2025Detalhes
Produtos recomendados
Nome do produto
MTT Formazan, MTT reduction end product
Formulário
powder
solubilidade
chloroform: 10 mg/mL, clear, dark violet
cadeia de caracteres SMILES
Cc1nc(\N=N\C(=N\Nc2ccccc2)c3ccccc3)sc1C
InChI
1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+
chave InChI
GIFKVQOPAJDJLO-SZVLODPRSA-N
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Ações bioquímicas/fisiológicas
MTT assay is a colorimetric reaction, generally used to examine cell viability. It involves the enzymatic reduction of a yellow-colored, water-soluble, 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to purple-colored, water-insoluble, MTT-formazan. This reaction is catalyzed by mitochondrial dehydrogenase and only occurs in viable cells.[4][5] Thus, the amount of blue formazan is proportional to the number of viable cells. The absorbance of the formazan solution is analyzed spectrophotometrically at 570 nm.[6]
Outras notas
Reviews: Tetrazolium salts and formazans[7]
produto relacionado
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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