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65906

Sigma-Aldrich

Phalloidin–Atto 647N

BioReagent, suitable for fluorescence, ≥80% (HPLC)

Sinônimo(s):

Atto 647N, Atto 647N-Phalloidin

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About This Item

Código UNSPSC:
12171501
NACRES:
NA.32
Preço e disponibilidade não estão disponíveis no momento.

linha de produto

BioReagent

Ensaio

≥80% (HPLC)

fabricante/nome comercial

ATTO-TEC GmbH

λ

in methanol

Absorção UV

λ: 640-646 nm Amax

adequação

suitable for fluorescence

método de detecção

fluorometric

temperatura de armazenamento

−20°C

Descrição geral

Phalloidin–Atto 647N is a new fluorescentlabel targeting the red spectral region. Atto 647N is a cationic dye, and postcoupling, it carries a net electrical charge of +1.Like most Atto labels, the absorption andfluorescence of Atto 647N are independent of pH between 2-11. Atto 647N issupplied in the form of a mixture containing two isomers having identicalfluorescence and absorption properties. Atto labels have rigid structures thatdo not show any cis-trans-isomerization.

Aplicação

Phalloidin–Atto 647Nis designed to be used for labelling DNA, RNA, or proteins. Fluorescentconjugates of phalloidin are used to label actin filaments for histologicalapplications. Some structural features of phalloidin are required for thebinding to actin.

Características e benefícios

Characteristic features of the Phalloidin Atto488 are:
  1. StrongAbsorption.
  2. HighFluorescence quantum yield.
  3. HighPhotostability.
  4. MinimalTriplet formation.
  5. GoodSolubility.
  6. Excellent Ozone Resistance.

Informações legais

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Pictogramas

Skull and crossbones

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral

Código de classe de armazenamento

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges


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Catherine Pfefferli et al.
Nature communications, 8, 15151-15151 (2017-05-04)
The existence of common mechanisms regulating organ regeneration is an intriguing concept. Here we report on a regulatory element that is transiently activated during heart and fin regeneration in zebrafish. This element contains a ctgfa upstream sequence, called careg, which
Georgios Trichas et al.
BMC biology, 6, 40-40 (2008-09-17)
Transgenic animals are widely used in biomedical research and biotechnology. Multicistronic constructs, in which several proteins are encoded by a single messenger RNA, are commonly used in genetically engineered animals. This is currently done by using an internal ribosomal entry
Benedetta Artegiani et al.
Nature cell biology, 22(3), 321-331 (2020-03-04)
CRISPR-Cas9 technology has revolutionized genome editing and is applicable to the organoid field. However, precise integration of exogenous DNA sequences into human organoids is lacking robust knock-in approaches. Here, we describe CRISPR-Cas9-mediated homology-independent organoid transgenesis (CRISPR-HOT), which enables efficient generation
Francesca Farina et al.
The EMBO journal, 38(11) (2019-04-25)
Cells going through mitosis undergo precisely timed changes in cell shape and organisation, which serve to ensure the fair partitioning of cellular components into the two daughter cells. These structural changes are driven by changes in actin filament and microtubule
Ceniz Zihni et al.
Nature cell biology, 19(9), 1049-1060 (2017-08-22)
Polarized epithelia develop distinct cell surface domains, with the apical membrane acquiring characteristic morphological features such as microvilli. Cell polarization is driven by polarity determinants including the evolutionarily conserved partitioning-defective (PAR) proteins that are separated into distinct cortical domains. PAR

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