This product may be stored at room temperature.
47671
Formamida
BioUltra, for molecular biology, ≥99.5% (T)
Sinônimo(s):
Amida C1, Amida fórmica
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About This Item
Fórmula linear:
HCONH2
Número CAS:
Peso molecular:
45.04
Beilstein:
505995
Número CE:
Número MDL:
Código UNSPSC:
12352111
ID de substância PubChem:
NACRES:
NA.25
Preço e disponibilidade não estão disponíveis no momento.
Produtos recomendados
grau
Molecular Biology
for molecular biology
densidade de vapor
1.55 (vs air)
pressão de vapor
0.08 mmHg ( 20 °C)
30 mmHg ( 129 °C)
linha de produto
BioUltra
Ensaio
≥99.5% (T)
Formulário
liquid
temperatura de autoignição
932 °F
técnica(s)
FISH: suitable
hybridization: suitable
protein extraction: suitable
Impurezas
DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
resíduo de ignição
≤0.1%
índice de refração
n20/D 1.447 (lit.)
n20/D 1.447
pH
7-9 (25 °C, 1 M in H2O)
p.e.
210 °C (lit.)
pf
2-3 °C (lit.)
solubilidade
H2O: 10 M at 20 °C, clear, colorless
densidade
1.134 g/mL at 25 °C (lit.)
traços de ânion
chloride (Cl-): ≤1 mg/kg
sulfate (SO42-): ≤10 mg/kg
traços de cátion
Ag: ≤5 mg/kg
Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
NH4+: ≤1000 mg/kg
Na: ≤20 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Tl: ≤5 mg/kg
Zn: ≤1 mg/kg
cadeia de caracteres SMILES
NC=O
λ
10 M in H2O
Absorção UV
λ: 260 nm Amax: ≤0.4
λ: 280 nm Amax: ≤0.1
aplicação(ões)
genomic analysis
InChI
1S/CH3NO/c2-1-3/h1H,(H2,2,3)
chave InChI
ZHNUHDYFZUAESO-UHFFFAOYSA-N
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Descrição geral
Formamide, also referred to as Carbamaldehyde, is an amide compound derived from formic acid. It plays a crucial role in biochemical research, particularly in denaturation and renaturation of nucleic acids (RNA and DNA). This includes applications like hybridization, sequencing gel electrophoresis, and electron microscopy. In molecular biology, BioUltra Formamide finds extensive use in various applications such as nucleic acid hybridization buffers, genetic fingerprinting, Southern and Northern blotting, as well as YAC screening. It typically functions by lowering the melting temperature of nucleic acid hybrids, facilitating their manipulation, and can enhance denaturation conditions in sequencing gels for more robust results.
Aplicação
A formamida desestabiliza as duplas de ácidos nucleicos e pode ser usada, tipicamente, em uma concentração de 50% em protocolos de hibridização que requerem temperatura de hibridização mais baixas.
Formamide has been used:
- as a component in the hybridization buffer for in situ hybridization as a component of solution X to wash the slides to remove the coverslips
- in the preparation of denaturation solution for spectral karyotyping (SKY) analysis
- in the preparation of 1% formaldehyde solution for the pretreatment of slides for comparative genomic hybridization (CGH)
- to prepare pre-hybridization buffer I, II, III, IV, and hybridization buffer I, II, and III for RNA fluorescence in situ hybridization (RNA-FISH)
- as an ionizing solvent in the production of formic esters and vitamin, a softener for paper, in animal glues, and water-soluble gums, in molecular biology as a denaturing agent
Ações bioquímicas/fisiológicas
Formamide serves as a good solvent for polar organic and inorganic compounds with potential catalytic activity in prebiotic processes due to its high dielectric constant.
Características e benefícios
- Versatile and adaptable for a wide variety of laboratory and research applications
- BioUltra Grade for your Molecular Biology, Cell Biology and Biochemical research
- Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
- Free from DNase, RNase, NICKase and proteaseTested for trace levels of Anions and Cations
Outras notas
For additional information on our range of Biochemicals, please complete this form.
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Carc. 2 - Repr. 1B - STOT RE 2 Oral
Órgãos-alvo
Blood
Código de classe de armazenamento
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
305.6 °F
Ponto de fulgor (°C)
152 °C
Equipamento de proteção individual
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
G. Wilkinson, ed.
In Situ Hybridization: A practical approach, 147-147 (1992)
R.C. Ogden et al.
Methods in Enzymology, 152, 67-67 (1987)
Min Chen et al.
Prenatal diagnosis, 35(6), 534-543 (2015-02-03)
To develop a single-tube multi-marker assay for improved preimplantation genetic diagnosis (PGD) of deletional and/or non-deletional Hb Bart's hydrops fetalis syndrome, providing haplotype confirmation of deletional status, and maximization of linkage informativity. We performed in silico mining to identify novel
Jing Wang et al.
The journal of physical chemistry. B, 117(8), 2314-2320 (2013-01-26)
A step-by-step mechanistic pathway following the transformation of formamide to purine through a five-membered ring intermediate has been explored by density functional theory computations. The highlight of the mechanistic route detailed here is that the proposed pathway represents the simplest
A Touati et al.
Journal of clinical microbiology, 53(10), 3182-3194 (2015-07-24)
Molecular typing of Mycoplasma pneumoniae is an important tool for identifying grouped cases and investigating outbreaks. In the present study, we developed a new genotyping method based on single nucleotide polymorphisms (SNPs) selected from the whole-genome sequencing of eight M.
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What temperature should this product be stored at?
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What temperature should this product be stored at?
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This product may be stored at room temperature.
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
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What expiry does Formamide get?
1 answer-
The product specification sheet for this product contains a recommended retest date period of roughly 11 months after the quality release date. A recommended retest date is the period of time during which the product is expected to remain within established stability specifications, provided that it has been stored under defined conditions. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications. For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the best way to deionize Formamide, Product 47671?
1 answer-
Mixed Bed Resin (e.g., Sigma Product No. M8157 or M8032) can be used to deionize formamide.
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What is the molarity of the neat liquid Formamide, Product 47671?
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Based on the density of 1.13 and the molecular weight of 45.04, the molarity of this neat liquid is 25.09 M. The pH of a 0.5 M aqueous solution is 7.1, according to the chemicals encyclopedia published by the Royal Society of Chemistry, 12th edition.
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Are aqueous solutions of Formamide, Product 47671, stable?
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In aqueous solution, formamide hydrolyzes to form ammonia and formic acid (i.e., ammonium formate).
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