318337

Acridine Orange hydrochloride hydrate

≥98% (HPLC)

• Sinônimo(s): 3,6-Bis(dimethylamino)acridine hydrochloride
• Fórmula empírica (Notação de Hill): C₁₇H₁₉N₃ · HCl · xH₂O
• Número CAS: 65-61-2
• Peso molecular: 301.81 (anhydrous basis)
• Número do índice de cores: 46005
• Beilstein: 3734978
• Número CE: 200-614-0
• Número MDL: MFCD00150070
• Código UNSPSC: 12171500
• ID de substância PubChem: 24859123
• NACRES: NA.47

Propriedades

• Nível de qualidade: 200
• Ensaio: ≥98% (HPLC)
• Formulário: powder
• técnica(s): microbe id | staining: suitable
• cor: orange
• pf: 284-287 °C (lit.)
• solubilidade: H₂O: 0.1%, clear, orange to red
• λmax: 492 nm
• ε (coeficiente de extinção): ≥14,000 at 227-233 nm in water at 0.005 g/L; ≥35000 at 265-271 nm in water at 0.005 g/L
• aplicação(ões): diagnostic assay manufacturing; hematology; histology
• temperatura de armazenamento: room temp
• cadeia de caracteres SMILES: Cl[H].[H]O[H].CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C
• InChI: 1S/C17H19N3.ClH.H2O/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;;/h5-11H,1-4H3;1H;1H2
• chave InChI: JUAAUEXLEKAEQC-UHFFFAOYSA-N

Descrição

Aplicação

A rigorously purified zinc-free material suitable for use in flow cytometry.

Acridine Orange hydrochloride hydrate dye has been used:

• to stain intracellular acidic vesicles.[1]
• to measure lysosomal membrane permeability (LMP).[1]
• or autophagy detection.[2]

Fluorescent stain for nucleic acids in agarose and polyacrylamide gels. Has been used to study apoptosis in various cell culture and tissue models.

Ações bioquímicas/fisiológicas

Acridine Orange is a metachromatic dye which can stain DNA, RNA and acid glycosaminoglycans. At low concentrations, it intercalates into DNA and precipitates RNA. However, at high concentrations it denatures and precipitates both RNA and DNA.[3] Acridine Orange is also used to analyze autophagy. It enters into acidic organelles in a pH-dependent manner. At neutral pH, acridine orange gives a green fluorescence and in acidic conditions, it accumulates in acidic organelles giving a bright red fluorescence.[4]

Produtos recomendados

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Warning
• Frases de perigo: H341
• Declarações de precaução: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Classificações de perigo: Muta. 2
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)