For staining a gel after electrophoresis, dilute a sample of the stock solution to 0.5 μg/mL with water and incubate the gel for 15-30 min. Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 μg/mL, and visualized immediately after electrophoresis.
E8751
Ethidium bromide
~95% (HPLC)
Sinônimo(s):
3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide, EtBr, Homidium bromide
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About This Item
Fórmula empírica (Notação de Hill):
C21H20BrN3
Número CAS:
Peso molecular:
394.31
Beilstein:
3642536
Número CE:
Número MDL:
Código UNSPSC:
41105322
ID de substância PubChem:
NACRES:
NB.22
Preço e disponibilidade não estão disponíveis no momento.
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Ensaio
~95% (HPLC)
Nível de qualidade
pf
260-262 °C (dec.) (lit.)
cadeia de caracteres SMILES
[Br-].CC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14
InChI
1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H
chave InChI
ZMMJGEGLRURXTF-UHFFFAOYSA-N
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Aplicação
Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.
Frameshift mutagen which intercalates double-stranded DNA and RNA.
Frameshift mutagen which intercalates double-stranded DNA and RNA.
Ações bioquímicas/fisiológicas
Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.
Reconstituição
For staining a gel after electrophoresis, dilute a sample of the stock solution to 0.5 μg/ml with water and incubate the gel for 15-30 min. Destaining is usually not needed but can be carried out in water for 15 min if decreased background is necessary. The DNA bands can then be detected on a UV light box (254 nm wavelength). Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 μg/ml and visualized immediately after electrophoresis.
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Descrição
Preços
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Acute Tox. 1 Inhalation - Acute Tox. 4 Oral - Muta. 2
Código de classe de armazenamento
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
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Conteúdo relacionado
Ethidium bromide is a well-known and widely used fluorescent dye in biotechnology research.
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What is the best working concentration of Product E8751, Ethidium bromide?
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How can I make a stock solution of Product E8751, Ethidium bromide?
1 answer-
Ethidium bromide is soluble in water at 100 mg/mL. Per Crary, B. and Boyrsenko, M., Biotechniques, 4, 98-101 (1986), stock solutions of ethidium bromide in water or PBS are stable for at least two years at room temperature if protected from light.
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How can I dispose of solutions containing Product E8751, Ethidium bromide?
1 answer-
Ethidium bromide is mutagenic, and ultimately has to be destroyed by incineration. It can be removed from buffer solutions by several methods to reduce the volume of waste. See the product sheet for more details.
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