RPOLT3-RO
Roche
T3 RNA Polymerase
from Escherichia coli HB101
Sinônimo(s):
mRNA, polymerase
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About This Item
Produtos recomendados
fonte biológica
Escherichia coli (HB101)
Nível de qualidade
Ensaio
100% (SDS-PAGE)
forma
solution
atividade específica
≥20 U/μL
peso molecular
100 kDa (single polypeptide chain)
embalagem
pkg of 1,000 U (11031163001)
pkg of 5,000 U (11031171001)
fabricante/nome comercial
Roche
concentração
<0.1 % (w/w)
técnica(s)
DNA sequencing: suitable
Northern blotting: suitable
Southern blotting: suitable
hybridization: suitable
cor
colorless
pH
7.9 (39 °F)
8.0 (68 °F)
solubilidade
water: miscible
adequação
suitable for molecular biology
nº de adesão NCBI
nº de adesão UniProt
aplicação(ões)
genomic analysis
life science and biopharma
atividade externa
Nicking activity 150 units, none detected
RNases 150 units
endonucleases 150 units, none detected
temperatura de armazenamento
−20°C
Informações sobre genes
Escherichia coli ... rpoB(948488)
Descrição geral
With this enzyme, homogeneously labeled single-stranded RNA can be generated. Transcripts can be nonradioactively labeled with biotin or DIG-11-UTP or radioactively labeled to high specific activity with [α-32P]- or [α-35S]-labeled nucleotides. T3 RNA polymerase is a DNA-dependent RNA polymerase that is highly specific for its promoter. Only DNA downstream from the T3 promoter will be transcribed.
Especificidade
Promotor specifity
T3 RNA polymerase is extremely promoter-specific and only transcribes bacteriophage T3 DNA or DNA cloned downstream of a T3 promoter.
Heat inactivation: Stop the reaction by adding 2 μl 0.2 M EDTA (pH 8.0) and/or heat to 65 °C.
T3 RNA polymerase is extremely promoter-specific and only transcribes bacteriophage T3 DNA or DNA cloned downstream of a T3 promoter.
Heat inactivation: Stop the reaction by adding 2 μl 0.2 M EDTA (pH 8.0) and/or heat to 65 °C.
Aplicação
T3 RNA Polymerase can transcribe RNA from cloned DNA templates that are downstream from a T3 promoter. The synthesis can be performed with labeled NTPs to generate highly labeled RNA. Synthesized RNA can be used in many applications, including:
- RNA or DNA blotting techniques
- In situ hybridization
- RNase protection studies: Transcripts synthesized by the enzyme are used as precursor RNA for studies on RNA splicing and processing.
- Synthesis of capped RNA in vitro with addition of m7GpppG or m7GpppA in excess over GTP or ATP during the transcription reaction. The generated antisense RNA can be introduced into cells to suppress the expression of the corresponding genes.
Embalagem
1 kit containing 2 components
Qualidade
Absence of endonucleases, nicking activity, and RNases tested according to the current Quality Control procedures. Function tested with the SP6/T7 Transcription Kit.
Especificações
Volume Activity: =20U/μl
Synthesis of hybridization probes: T3 RNA polymerase allows highly efficient production of homogeneously labeled RNA. This labeled RNA may be used as hybridization probes in Southern, northern, and dot blots, as well as in situ hybridizations.
Suitable labels: Transcripts can be nonradioactively labeled with biotin-16-UTP, DIG-11-UTP, or fluorescein-12-UTP. They may also be radioactively labeled to high specific activity with [a-32P]- or [a-35S]-labeled nucleotides.
Note: Roche has 10x concentrated RNA Labeling Mixes that are specially designed for DIG-, biotin-, and fluorescein-labeling. These mixes work well with T3 RNA Polymerase.
Synthesis of hybridization probes: T3 RNA polymerase allows highly efficient production of homogeneously labeled RNA. This labeled RNA may be used as hybridization probes in Southern, northern, and dot blots, as well as in situ hybridizations.
Suitable labels: Transcripts can be nonradioactively labeled with biotin-16-UTP, DIG-11-UTP, or fluorescein-12-UTP. They may also be radioactively labeled to high specific activity with [a-32P]- or [a-35S]-labeled nucleotides.
Note: Roche has 10x concentrated RNA Labeling Mixes that are specially designed for DIG-, biotin-, and fluorescein-labeling. These mixes work well with T3 RNA Polymerase.
Definição da unidade
One unit is the enzyme activity which incorporates 1 nMol CMP in acid-precipitable RNA products within 60 minutes at +37 °C.
Volume Activity: ≥20 U/μl
Volume Activity: ≥20 U/μl
Nota de preparo
Activator: BSA/spermine
Armazenamento e estabilidade
Keep container tightly closed in a dry and well-ventilated place.
Outras notas
For life science research only. Not for use in diagnostic procedures.
Somente componentes do kit
Nº do produto
Descrição
- T3 RNA Polymerase, in buffer, pH 7.9 ≥20 U/μl
- Transcription Buffer 10x concentrated
Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Classificações de perigo
Eye Irrit. 2
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
does not flash
Ponto de fulgor (°C)
does not flash
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