04716728001
Roche
DNase I recombinant, RNase-free
from bovine pancreas, expressed in Pichia pastoris
About This Item
Produtos recomendados
fonte biológica
bovine pancreas
Nível de qualidade
recombinante
expressed in Pichia pastoris
forma
solution
peso molecular
~39 kDa
embalagem
pkg of 10,000 U
fabricante/nome comercial
Roche
pH ideal
7.0-8.0
Descrição geral
Contents
- Recombinant DNase I, RNase-free, 10 U/μl
- Incubation Buffer, 10x concentrated
Especificidade
Important Note: Alternatively, DNase I recombinant, RNase-free can be inactivated and removed by phenol extraction according to standard protocols, e.g., Current Protocols in Molecular Biology.
Aplicação
- Remove genomic DNA from RNA preparations prior to RT-PCR
- Isolate DNA-free RNA after in vitro transcription reactions
- Perform nick translations
- Map DNase-sensitive regions in eukaryotic DNA
Características e benefícios
- Eliminates DNA contamination from any RNA sample
- Contains no detectable RNase or protease activity
- Can be heat inactivated, thereby eliminating the need for organic extraction
- Shipped with an optimized incubation buffer, which supports maximum DNase activity
- Produced via an entirely animal-free process, to eliminate any risks associated with animal-derived material
Embalagem
Qualidade
Especificações
Recombinant DNase I is heterogeneously N-glycosylated, so it appears as two bands in gel electrophoresis.
Divalent ion requirement
DNase I requires divalent cations for maximum activity. The DNA-specific endonuclease is activated by ions such as magnesium ions and is stimulated by calcium ions. Therefore, the enzyme is inhibited by metal chelating agents like EDTA.
Definição da unidade
Assay conditions:
Volume activity is determined according to the following assay mixture. 100 μg calf thymus DNA is incubated in 2.5 ml 1x incubation buffer with 40 to 70 units DNase I recombinant, RNase-free at +25 °C. The absorbance increase is measured at 260 nm.
Nota de preparo
Working solution: Storage Buffer: 20 mM Tris-HCl, 50 mM NaCl, 2 mM CaCl2, 2 mM MgCl2, 1 mM dithioerythritol, 0.1 mg/ml Pefabloc SC, 50% glycerol (v/v), pH 7.6 (at 4 °C).
Incubation Buffer (10x): 400 mM Tris-HCl, 100 mM NaCl, 60 mM MgCl2, 10 mM CaCl2, pH 7.9.
Enzyme Dilution Buffer: 25 mM Tris-HCl, 50% glycerol (v/v), pH 7.6 (at 4 °C).
Armazenamento e estabilidade
Outras notas
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
does not flash
Ponto de fulgor (°C)
does not flash
Certificados de análise (COA)
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Protocolos
DNase I from bovine pancreas is a glycoprotein of Mr 37000. A special procedure is used to remove RNases from the DNase preparation.
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