11633716001
Roche
Anti-Digoxigenin-POD (poly), Fab fragments
from sheep
Sinônimo(s):
anti-digoxigenin, digoxigenin
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About This Item
Código UNSPSC:
12352203
Preço e disponibilidade não estão disponíveis no momento.
Produtos recomendados
fonte biológica
sheep
Nível de qualidade
conjugado
peroxidase conjugate
forma do anticorpo
purified immunoglobulin
tipo de produto de anticorpo
primary antibodies
clone
polyclonal
Formulário
lyophilized
embalagem
pkg of 50 U
fabricante/nome comercial
Roche
Isotipo
IgG
temperatura de armazenamento
2-8°C
Categorias relacionadas
Descrição geral
Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems.[1] Probes labeled with digoxigenin has greater sensitivity equivalent to that of radioactive probes, allows faster detection. It is less hazardous and has increased shelf life.[2] This product contains Fab fragments from polyclonal anti-digoxigenin antibodies, conjugated to polymerized horseradish peroxidase.
Especificidade
The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.
Aplicação
Anti-Digoxigenin-POD (poly), Fab fragments has been used for the detection of digoxigenin-labeled compounds using:
Anti-Digoxigenin-POD (poly), Fab fragments give considerably higher signal-to-noise values compared to anti-Digoxigenin-Fab fragment conjugated to the unpolymerized horseradish peroxidase in ELISA applications. It is specifically useful when high sensitivity is required.
Anti-Digoxigenin-POD (poly), Fab fragments have not been evaluated in immunohistochemistry.
- whole-mount in situ hybridization[3]
- fluorescence in situ hybridization[4]
- enzyme linked immunosorbent assay (ELISA)[5]
- dot blot
- Southern blot
- western blot
- double labeling experiments
- lectin blots
Anti-Digoxigenin-POD (poly), Fab fragments give considerably higher signal-to-noise values compared to anti-Digoxigenin-Fab fragment conjugated to the unpolymerized horseradish peroxidase in ELISA applications. It is specifically useful when high sensitivity is required.
Anti-Digoxigenin-POD (poly), Fab fragments have not been evaluated in immunohistochemistry.
Nota de preparo
Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:
Working solution: 100 mM Tris-HCl, 150 mM NaCl, pH 7.5.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.
- Dot blot: 50 to 100 mU/ml
- ELISA: 2 to 50 mU/ml
- Western blot: 50 to 100 mU/ml
- Southern blot: 50 to 100 mU/ml
Working solution: 100 mM Tris-HCl, 150 mM NaCl, pH 7.5.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.
Reconstituição
Add 1 ml double-distilled water to a final concentration of 50 U/ml.
Outras notas
For life science research only. Not for use in diagnostic procedures.
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Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Classificações de perigo
Skin Sens. 1
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
does not flash
Ponto de fulgor (°C)
does not flash
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Artigos
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
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