Pular para o conteúdo
Merck
Todas as fotos(2)

Documentos

11585762001

Roche

DIG Wash and Block Buffer Set

greener alternative

storage temp.:2-8°C

Sinônimo(s):

wash and block buffer set, dig

Faça loginpara ver os preços organizacionais e de contrato
Todas as fotos(2)

About This Item

Código UNSPSC:
41105326

Nível de qualidade

100

fabricante/nome comercial

Roche

características do produto alternativo mais ecológico

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

categoria alternativa mais ecológica

, Aligned

temperatura de armazenamento

2-8°C

Categorias relacionadas

Descrição geral

Except for the Blocking solution, the 10x concentrated buffers are diluted to the desired concentrations with sterile double-distilled water (not included).
Washing, blocking and detection buffers (10x concentrated) for the immunological detection of DIG - (digoxigenin-)labeled probes.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Aplicação

The DIG Wash and Block Buffer Set is used at various steps of DIG hybridization and DIG detection.

Embalagem

1 set containing 4 components.

Qualidade

The buffers are autoclaved, filtered and tested for the absence of DNases and RNases according to the current Quality Control procedures. The buffers are function tested according to the standard procedure of the DIG Nucleic Acid Detection Kit.

Nota de preparo

Working solution: dATP, dGTP, dTTP mixture:
For one labeling reaction pipette:
1 μl dATP, (vial 2)
1 μl dGTP, (vial 4)
1 μl dTTP, (vial 5)
to a reaction vial.

Note: If the same type of labeled deoxyribonucleoside-triphosphate is used repeatedly, we recommend the preparation of a mixture of equal parts of the other three deoxyribonucleoside-triphosphates for convenience.
Storage conditions (working solution):
  • After the first usage, the buffers should be stored further at 2 to 8 °C. We recommend to store the concentrated Blocking solution in aliquots at -15 to -25 °C.
  • When Autoclaved: Stock solution can be stored for several days to a week either unopened at RT or at 2 to 8 °C after opening. Alternatively, it can be stored in aliquots at -15 to -25 °C for up to 6 months.

Outras notas

For life science research only. Not for use in diagnostic procedures.

Somente componentes do kit

Nº do produto
Descrição
  • Washing Buffer 10x concentrated
  • Maleic acid Buffer 10x concentrated
  • Blocking Solution 10x concentrated
  • Detection Buffer 10x concentrated

Pictogramas

Exclamation mark
GHS07

Palavra indicadora

Warning

Frases de perigo

H315,H319

Classificações de perigo

Eye Irrit. 2 - Skin Irrit. 2

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicabledoes not flash

Ponto de fulgor (°C)

Not applicabledoes not flash

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Shiho Torii et al.
Cell reports, 35(3), 109014-109014 (2021-04-12)
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been identified as the causative agent of coronavirus disease 2019 (COVID-19). Although multiple mutations have been observed in SARS-CoV-2, functional analysis of each mutation of SARS-CoV-2 has been limited by the lack
James A Baum et al.
Journal of economic entomology, 105(2), 616-624 (2012-05-23)
The plant bugs Lygus hesperus Knight (Hemiptera: Miridae) and L. lineolaris (Palisot de Beauvois) have emerged as economic pests of cotton in the United States. These hemipteran species are refractory to the insect control traits found in genetically modified commercial
Dan Sun et al.
Oncology letters, 20(4), 8-8 (2020-08-11)
Since human bladder cancer (BC) is a common malignancy of the urinary system with poor prognosis, it is crucial to clarify the molecular mechanisms of BC development and progression. To the best of our knowledge, the current study demonstrated for
Siqian Feng et al.
Genetics, 217(3) (2021-03-28)
We describe a simple and efficient technique that allows scarless engineering of Drosophila genomic sequences near any landing site containing an inverted attP cassette, such as a MiMIC insertion. This two-step method combines phiC31 integrase-mediated site-specific integration and homing nuclease-mediated
James V McCann et al.
Genesis (New York, N.Y. : 2000), 58(7), e23369-e23369 (2020-06-17)
Extracellular vesicles (EVs) are abundant, lipid-enclosed vectors that contain nucleic acids and proteins, they can be secreted from donor cells and freely circulate, and they can be engulfed by recipient cells thus enabling systemic communication between heterotypic cell types. However
Exibir todos os documentos relacionados

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica