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12041677001

Roche

CDP-Star®, ready-to-use

>98%, solution, suitable for dot blot, suitable for Northern blotting, suitable for Southern blotting

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About This Item

Código UNSPSC:
12352204

descrição

Disodium 2-chloro-5-(4-methoxyspiro {1,2-dioxetane-3,2′-(5′-chloro)tricyclo[3.3.1.13,7 ]decan}-4-yl)-1-phenyl phosphate

Nível de qualidade

100

Ensaio

>98%

Formulário

solution

embalagem

bottle of 2 × 50 mL ([0.25 mM])

fabricante/nome comercial

Roche

técnica(s)

Northern blotting: suitable
Southern blotting: suitable
dot blot: suitable

temperatura de armazenamento

2-8°C

Publicação

Purity: Purified by HPLC.

Aplicação

Used for the nonradioactive detection of labeled nucleic acid probes on membrane blots:
  • Southern blot
  • Northern blot
  • Dot blot
  • Colony and plaque hybridization
  • Gel shift assays
Used for the nonradioactive detection of labeled nucleic acid probes on membrane blots:
  • Southern blot
  • Northern blot
  • Dot blot
  • Colony and plaque hybridization
  • Gel shift assays
  • Chemiluminescent detection.

Características e benefícios

Contents
0.25 mM solution (0.124 mg/ml), ready-to-use, colorless solution
CDP-Star is a chemiluminescent substrate for alkaline phosphatase that enables fast, ultra-sensitive detection of biomolecules by producing extremely rapid visible light. Light emission is recorded on X-ray film or on luminescence imager systems.
  • Save time with a fast, ultra-sensitive substrate.
  • Easily strip and reprobe membranes.
  • Take multiple exposures of up to two days for each experiment.
Reuse the substrate by filtering and storing in sodium azide.

Nota de preparo

Storage conditions (working solution): Washing Buffer: 15 to 25 °C, stable
Maleic Acid Buffer: 15 to 25 °C, stable
Detection Buffer: 15 to 25 °C, stable
Blocking Solution: always prepare freshly
Antibody Solution: 2 to 8 °C

Outras notas

  • First exposure to X-ray film should be 5-15 minutes. Examine film and then determine other exposure times based on initial result.
  • No preincubation step required.
  • Apply substrate quickly to avoid gray shadows from substrate drops and drying of membrane (leads to uneven, high background).
  • Do not use plastic wrap to cover blot; use hybridization bags, acetate sheet protectors, or two sheets of transparent film.
  • Exposure times are shorter than with CSPD.
  • Do not use nitrocellulose membranes.
Repeat exposures can be made up to two days after the addition of substrate.
For life science research only. Not for use in diagnostic procedures.

Informações legais

CDP-Star is a registered trademark of Tropix, Inc.

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

nwg

Ponto de fulgor (°F)

does not flash

Ponto de fulgor (°C)

does not flash

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Detection of expanded RNA repeats using thermostable group II intron reverse transcriptase.
Carrell ST, et al.
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