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Documentos Principais

11796895001

Roche

DIG Easy Hyb Granules

greener alternative

powder, suitable for hybridization

Sinônimo(s):

DIG system

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About This Item

Código UNSPSC:
41105326

Agency

suitable for

Nível de qualidade

Formulário

powder

uso

sufficient for 6 x 100 mL

fabricante/nome comercial

Roche

condição de armazenamento

(Keep container tightly closed in a dry and well-ventilated place.)

características do produto alternativo mais ecológico

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

técnica(s)

hybridization: suitable

cor

white

pH

7,0

solubilidade

water: soluble

adequação

suitable for nucleic acid hybridization

categoria alternativa mais ecológica

temperatura de armazenamento

20-25°C

Descrição geral

DIG Easy Hyb is a hybridization solution used for nucleic acid blots with digoxigenin-labeled probes. DIG Easy Hyb does not contain formamide but facilitates the lowering of hybridization temperature comparable to buffers that contain 50% formamide.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Aplicação

DIG Easy Hyb Granules can be used in all membrane hybridization applications using nonradioactive (e.g., digoxigenin-labeled) nucleic acid probes. It is specifically designed for use with DIG kits.

Características e benefícios

  • DIG Easy Hyb is a convenient ready-to-use hybridization buffer, saving time and effort.
  • DIG Easy Hyb aligns with the principles of Green Chemistry by offering a greener alternative for hybridization buffers.

Nota de preparo

Working solution: Add carefully 64 ml sterile double-dist. water to the plastic bottle, dissolve by stirring immediately for 5 minutes at 37 °C. The availability of 6 x 100 ml portions allows you to freshly "produce" the DIG Easy Hyb when required—no long-term storage of a solution is required.
Note: For applications with Northern blots, DIG Easy Hyb Granules must be dissolved under RNase-free conditions.
Nontoxic granules, aliquoted in plastic bottles. Reconstitute to provide 100ml of a 1x solution, ready-to-use.

Outras notas

For life science research only. Not for use in diagnostic procedures.

Informações legais

DIG Easy Hyb is a trademark of Roche

Pictogramas

Exclamation mark

Palavra indicadora

Warning

Frases de perigo

Classificações de perigo

Eye Irrit. 2 - Skin Irrit. 2

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

does not flash

Ponto de fulgor (°C)

does not flash


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Protocolos

Protocol for DIG Easy Hyb™ Granules Protocol & Troubleshooting

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