8.51025

5-Carboxyfluorescein

Novabiochem^®

• Sinônimo(s): 5-Carboxyfluorescein, 5-FAM
• Fórmula empírica (Notação de Hill): C₂₁H₁₂O₇
• Número CAS: 76823-03-5
• Peso molecular: 376.32
• Número MDL: MFCD00036874
• Código UNSPSC: 12352005
• NACRES: NA.22

Propriedades

• Nível de qualidade: 200
• linha de produto: Novabiochem^®
• Ensaio: ≥98% (TLC); ≥99.0% (HPLC)
• forma: powder
• adequação da reação: reagent type: fluorescent-labelling reagent
• fabricante/nome comercial: Novabiochem^®
• aplicação(ões): peptide synthesis
• temperatura de armazenamento: 2-8°C
• InChI: 1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)
• chave InChI: NJYVEMPWNAYQQN-UHFFFAOYSA-N

Descrição

Descrição geral

This high purity fluorescent dye (λ_ex494 nm, λ_em 518 nm) is supplied as a single isomer, which ensures production of labeled products of defined chemical structure, as well as greatly simplifying purification and characterization. FAM should be introduced as the last step in the synthesis to avoid formation of side-products, or be protected by tritylation prior to acylation or hydrazine treatment ^[1].

Associated Protocols and Technical Articles
Chromogenic Labeling of Synthetic Peptides

Literature references

^[1] R. Fischer, et al. (2003) Bioconjugate Chem., 14, 653.

Aplicação

5-Carboxyfluorescein Novabiochem^®. CAS 76823-03-5, molar mass 376.32 g/mol.

Ligação

Replaces: 01-63-0112

Nota de análise

Colour (visual): yellow to orange
Appearance of substance (visual): powder
Identity (IR): passes test
Purity (TLC (EtOAc/MeOH/H2O/DIPEA 60 : 30 : 10 : 3)): ≥ 98 %
Assay (HPLC, area%): ≥ 99.0 % (a/a)

To see the solvent systems used for TLC of Novabiochem^® products please click here.

Informações legais

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable