C2734

5-Carboxytetramethylrhodamine

≥95% purity (HPLC), solid

• Sinônimo(s): 5-TAMRA
• Fórmula empírica (Notação de Hill): C₂₅H₂₂N₂O₅
• Número CAS: 91809-66-4
• Peso molecular: 430.45
• Número MDL: MFCD01073638
• Código UNSPSC: 12171500
• NACRES: NA.47

Propriedades

• Nome do produto: 5-Carboxytetramethylrhodamine,
• Ensaio: ≥95% (HPLC)
• Nível de qualidade: 100
• Formulário: solid
• solubilidade: water: slightly soluble
• ε (coeficiente de extinção): ≥85000-97000 at 539-545 nm in methanol
• fluorescência: λ_ex ~543 nm; λ_em ~570 nm in methanol
• aplicação(ões): diagnostic assay manufacturing; hematology; histology
• temperatura de armazenamento: room temp
• cadeia de caracteres SMILES: N(C)(C)c1cc2[o+]c3c(c(c2cc1)c4c(cc(cc4)C(=O)[O-])C(=O)O)ccc(c3)N(C)C
• InChI: 1S/C25H22N2O5/c1-26(2)15-6-9-18-21(12-15)32-22-13-16(27(3)4)7-10-19(22)23(18)17-8-5-14(24(28)29)11-20(17)25(30)31/h5-13H,1-4H3,(H-,28,29,30,31)
• chave InChI: YMZMTOFQCVHHFB-UHFFFAOYSA-N

Descrição

Descrição geral

An important fluorophore prepared in high purtity with a detection limit of TMR-labeled amino acids by capillary electrophoresis of approx. 600 molecules. Readily excited by the 546 nm spectral line from mercury-arc lamps used in fluorescent microscopes, and is intrinsically more photostable than fluorescein.

Aplicação

5-Carboxytetramethylrhodamine has been used in fluorophore characterization.[1]

Ações bioquímicas/fisiológicas

5-Carboxytetramethylrhodamine can be covalently attached to the DNA and is thereby used in studies involving branched and duplex DNA/RNA structures.[2] It is mainly used for single-molecule studies.[3] 5-Carboxytetramethylrhodamine is also used for the labelling of oligosaccharide for laser-induced fluorescence detection.[4]

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)