46950
Fluorescein 5(6)-isothiocyanate
BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)
Sinônimo(s):
FITC, Fluorescein isothiocyanate
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About This Item
Fórmula empírica (Notação de Hill):
C21H11NO5S
Número CAS:
Peso molecular:
389.38
Beilstein:
1407295
Número CE:
Número MDL:
Código UNSPSC:
12171500
ID de substância PubChem:
NACRES:
NA.32
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Nível de qualidade
linha de produto
BioReagent
Ensaio
≥90% (HPLC)
fluorescência
λex 492 nm; λem 518 nm in 0.1 M phosphate pH 8.0
adequação
suitable for fluorescence
temperatura de armazenamento
−20°C
cadeia de caracteres SMILES
Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
InChI
1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
chave InChI
MHMNJMPURVTYEJ-UHFFFAOYSA-N
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Descrição geral
Fluorescein 5(6)-isothiocyanate, also known as FITC, is a weakly acidic hydroxyxanthene whose overall size is small, with both carboxylic acid and isothiocyanate substituents on the pendant phenyl ring. The dye has a moderately sized, nonplanar aromatic system. The isothiocyanate group reacts with nucleophilic substituents (e.g. amino, hydroxyl, thiol) of biomolecules to be attached as a fluorescent label. The maximum emission of the dye is at 519nm in the same solvent.
Aplicação
Fluorescein 5(6)-isothiocyanate (FITC) labelled molecules are used in non-microscopic immunoassays and physiological tracing experiments. FITC is widely used in flow cytometry experiments for staining proteins, nuclei, and subnuclear particles. White blood cells (WBCs) are marked with FITC to study their response toward a chemoattractant, eventually being measured.
For fluorescent labeling of proteins (e.g. immunofluorescence); H Protein labeling for gel chromatography
Embalagem
Bottomless glass bottle. Contents are inside inserted fused cone.
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Equipamento de proteção individual
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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