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D5921

Sigma-Aldrich

DMEM - low glucose

With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

Synonyme(s) :

DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM

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About This Item

Numéro MDL:
MFCD00217342
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75

product name

Dulbecco′s Modified Eagle′s Medium - low glucose, With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

Stérilité

sterile-filtered

Forme

liquid

Technique(s)

cell culture | mammalian: suitable

Impuretés

endotoxin, tested

Composants

phenol red: no
sodium pyruvate: no
glucose: low
HEPES: no
L-glutamine: no
NaHCO3: yes

Conditions d'expédition

ambient

Température de stockage

2-8°C

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Catégories apparentées

Description générale

Dulbecco′s Modified Eagle′s medium (DME) is a modification of Basal Medium Eagle (BME), which has a four-fold higher concentration of amino acids and vitamins along with additional supplementary components. The first original DME formula was reported to culture embryonic mouse cells comprising 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types. DME has been shown to improve the stability of the medium.

Application

Dulbecco′s Modified Eagle′s medium-low glucose has been used to culture:
  • living colon cancer SW620 cells
  • human breast carcinoma cell lines MCF-7(13), KPL and MDA-MB-231
  • mammal and canine derived adipose-derived mesenchymal stem cells

Reconstitution

Supplement with 0.584 g/L L-glutamine.

Autres remarques

Investigators who work with stem cells or cell clones frequently prefer media without phenol red.

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Tarif

Supplément

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Description
Tarif

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H317

Classification des risques

Skin Sens. 1

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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C Mignon et al.
Scientific reports, 7(1), 2797-2797 (2017-06-07)
Photobiomodulation-based (LLLT) therapies show tantalizing promise for treatment of skin diseases. Confidence in this approach is blighted however by lamentable inconsistency in published experimental designs, and so complicates interpretation. Here we interrogate the appropriateness of a range of previously-reported treatment
Fabian E Ortega et al.
eLife, 8 (2019-02-06)
Listeria monocytogenes hijacks host actin to promote its intracellular motility and intercellular spread. While L. monocytogenes virulence hinges on cell-to-cell spread, little is known about the dynamics of bacterial spread in epithelia at a population level. Here, we use live
Christina Schmid et al.
Journal of pharmacological and toxicological methods, 106, 106915-106915 (2020-09-02)
Human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes are being evaluated for their use in pharmacological and toxicological testing, particularly for electrophysiological side effects. However, little is known about the composition of the commercially available iCell cardiomyocyte (Fuijifilm Cellular Dynamics) cultures
Philip H Choi et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(35), E7226-E7235 (2017-08-16)
Cyclic di-3',5'-adenosine monophosphate (c-di-AMP) is a broadly conserved bacterial second messenger that has been implicated in a wide range of cellular processes. Our earlier studies showed that c-di-AMP regulates central metabolism in Listeria monocytogenes by inhibiting its pyruvate carboxylase (LmPC)
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