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V900412
Tricin
Vetec™, reagent grade, ≥99%
Synonym(e):
N-[Tris-(hydroxymethyl)-methyl]-glycin
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About This Item
Lineare Formel:
(HOCH2)3CNHCH2CO2H
CAS-Nummer:
Molekulargewicht:
179.17
Beilstein:
1937804
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12161700
PubChem Substanz-ID:
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Empfohlene Produkte
Qualität
reagent grade
Produktlinie
Vetec™
Assay
≥99%
Form
crystalline powder
Nützlicher pH-Bereich
7.4-8.8
pKa (25 °C)
8.1
SMILES String
OCC(CO)(CO)NCC(O)=O
InChI
1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)
InChIKey
SEQKRHFRPICQDD-UHFFFAOYSA-N
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Allgemeine Beschreibung
Tricine is the most used electrophoresis buffer, which has a lower negative charge than glycine. This in turn helps it to migrate faster. Tricine is also used for the resuspension of cell pellets. Moreover, its high ionic strength leads to more movement of ions and less protein movement. This aids to separate low molecular weight proteins in lower percent acrylamide gels. Tricine is also used as a trailing ion that helps in the resolution of small proteins at lower acrylamide concentrations compared to glycine-sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) systems.[1]
Anwendung
Pufferkomponente für die Trennung von Peptiden mit geringer Molekülmasse.
Rechtliche Hinweise
Vetec is a trademark of Merck KGaA, Darmstadt, Germany
Lagerklassenschlüssel
13 - Non Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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Electrophoretic separations of proteins are widely used in proteomic analyses, and rely heavily on SDS electrophoresis. This mode of separation is almost exclusively used when a single dimension separation is performed, and generally represents the second dimension of two-dimensional separations.
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Totally porous lipid-based liquid crystalline nanoparticles were used as pseudostationary phase for capillary electroseparation with LIF detection of proteins at physiological conditions using unmodified cyclic olefin copolymer capillaries (Topas, 6.7 cm effective length). In the absence of nanoparticles, i.e. in
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Nature protocols, 1(1), 16-22 (2007-04-05)
Tricine-SDS-PAGE is commonly used to separate proteins in the mass range 1-100 kDa. It is the preferred electrophoretic system for the resolution of proteins smaller than 30 kDa. The concentrations of acrylamide used in the gels are lower than in
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