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T0377

Tricin

≥99% (titration)

Synonym(e):

N-[Tris-(hydroxymethyl)-methyl]-glycin

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Größe/SKUVerfügbarkeitPreis
25 g
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€ 50,50
100 g
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€ 174,00
250 g
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€ 332,00
1 kg
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€ 945,00
5 kg
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€ 3.480,00
25 kg
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€ 13.260,00

Über diesen Artikel

Lineare Formel:
(HOCH2)3CNHCH2CO2H
CAS-Nummer:
Molekulargewicht:
179.17
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
EC Number:
227-193-6
Beilstein/REAXYS Number:
1937804
MDL number:

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Quality Segment

assay

≥99% (titration)

form

crystalline powder

technique(s)

SDS-PAGE: suitable

impurities

≤5 ppm Heavy Metals (as Lead)

useful pH range

7.4-8.8

pKa (25 °C)

8.1

mp

187 °C

solubility

water: 0.25 g/mL, clear, colorless

suitability

suitable for electrophoresis

application(s)

diagnostic assay manufacturing

SMILES string

OCC(CO)(CO)NCC(O)=O

InChI

1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)

InChI key

SEQKRHFRPICQDD-UHFFFAOYSA-N

General description

Tricin ist der am häufigsten eingesetzte Elektrophoresepuffer. Er ist auch nützlich für die Resuspension von Zellrückständen. Tricin dient als Folgeion und unterstützt die Auflösung von kleinen Proteinen bei geringeren Acrylamidkonzentrationen als in SDS-PAGE-Systemen (sodium dodecyl-sulfate polyacrylamide gel electrophoresis) mit Glycin. Es weist eine schwächere negative Ladung als Glycin auf, wodurch es schneller migriert. Tricin besitzt eine hohe Ionenstärke, die eine bessere Ionenbewegung und geringere Proteinbewegung ermöglicht. Dies führt wiederum zur Abscheidung von Proteinen mit geringer Molekülmasse in Gelen mit geringerem Acrylamidanteil.

Application

Tricin wird wie folgt verwendet:
  • zum Verhindern der Ausfällung von Salzen während des Autoklavierens von Emiliana huxleyi-Kulturen
  • als Komponente von Puffer A zum Homogenisieren von Proben aus beispielsweise Caenorhabditis elegans, Drosophila und Pflanzen
  • als Komponente von frischem Assay-Puffer zum Messen von Serummelatonin durch Radioimmunassays

Pufferkomponente für die Trennung von Peptiden mit geringer Molekülmasse.

Features and Benefits

Features:
  1. Qualified Raw Materials: Products that meet the stringent requirements of the Elevated Assurance Program, ensuring compliance with regulatory standards.
  2. Comprehensive Documentation: Includes essential documents such as certificates, impurity profiles, and declarations to support audits and risk assessments.
  3. M-Clarity Quality Attributes: Products are classified within the MQ300 quality segment or higher, demonstrating superior quality assurance. M-Clarity Program
  4. Confirmed Process Controls: Established processes that ensure consistency and reliability in manufacturing.
  5. Enhanced Change Notification: Timely updates on any changes to product specifications or processes, ensuring transparency and compliance.
  6. Freely Available Dossiers: Access to robust, ready-to-present product dossiers that include:
    1. Site quality assessment
    2. ISO 9001 certificate
    3. Detailed product specifications
    4. Relevant declarations/statements (e.g., animal origin content, BSE/TSE, nitrosamine)
Benefits:
  1. Regulatory Compliance: Helps you meet compliance standards effectively, reducing the risk of regulatory issues.
  2. Audit Readiness: Instills confidence during audits with comprehensive documentation and confirmed process controls, demonstrating your commitment to high-quality products.
  3. Operational Transparency: Supports supply chain transparency, allowing you to track and verify the quality of raw materials.
  4. Enhanced Support: Provides exceptional quality-related information and tailored assistance to meet your specific needs.
  5. Risk Mitigation: Facilitates thorough risk assessments with detailed documentation, helping you manage potential compliance challenges.
  6. Informed Decision-Making: Access to clear and comprehensive product information enables better decision-making and enhances operational efficiency.

Legal Information

M-CLARITY is a trademark of Merck KGaA, Darmstadt, Germany

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Dieser Artikel
T17917137262RDD024
Tricin EMPROVE® EVOLVE

SAFC

137262

Tricin

description

≥99% (titration)

description

≥99% (titration), reagent grade, crystalline

description

EMPROVE® EVOLVE

description

anhydrous, free-flowing, Redi-Dri, ≥99%

assay

≥99% (titration)

assay

≥99% (titration)

assay

-

assay

≥99%

technique(s)

SDS-PAGE: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

technique(s)

-

Quality Level

400

Quality Level

200

Quality Level

400

Quality Level

100

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

-

application(s)

-

form

crystalline powder

form

crystalline

form

powder

form

powder

solubility

water: 0.25 g/mL, clear, colorless

solubility

water: 0.1 M, clear, colorless

solubility

-

solubility

water: 20 % (w/w), clear, colorless


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Lagerklasse

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Analysenzertifikate (COA)

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Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

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