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Ascentis® Express 90 Å Phenyl-Hexyl (2.7 μm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

Synonym(e):

Core-shell-(SPP-)Fused-Core-Phenyl-HPLC-Säule

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About This Item

UNSPSC-Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
Preise und Verfügbarkeit sind derzeit nicht verfügbar.

Produktbezeichnung

Ascentis® Express Phenyl-Hexyl, 2,7 μm HPLC-Säule, 2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

Materialien

stainless steel column

Agentur

suitable for USP L11

Produktlinie

Ascentis®

Leistungsmerkmale

endcapped

Hersteller/Markenname

Ascentis®

Verpackung

1 ea of

Parameter

60 °C temp. range
600 bar max. pressure (9000 psi)

Methode(n)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

L × ID

15 cm × 4.6 mm

Oberflächenbereich

135 m2/g

Verunreinigungen

<5 ppm metals

Matrix

Fused-Core particle platform
superficially porous particle

Aktive Matrixgruppe

phenylhexyl phase

Partikelgröße

2.7 μm

Porengröße

90 Å

pH-Bereich

2-9

Anwendung(en)

food and beverages

Trenntechnik

reversed phase

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Verwandte Kategorien

Allgemeine Beschreibung

Die Phenyl-Hexyl-Phase weist durch die Wechselwirkung zwischen der gelösten Substanz und dem aromatischen Ring und seinen delokalisierten Elektronen eine einzigartige Selektivität auf. Aufgrund dieser einzigartigen Aromatizität ist sie komplementär (orthogonal) zur C18- und RP-Amid-Phase. Des Weiteren neigt die Phenyl-Hexyl-Phase zu einer guten Formselektivität, die vermutlich aus der Mehrfachwechselwirkung zwischen der gelösten Substanz und dem planaren Ringsystem resultiert. Bei gelösten Stoffen mit aromatischen elektronenziehenden Gruppen (Fluor, Nitro usw.) oder mit einem delokalisierten heterozyklischen Ringsystem, wie bei Benzodiazepin-Verbindungen, wird häufig eine höhere Retention und Selektivität beobachtet.

Anwendung

  • A Different Perspective on the Characterization of a New Degradation Product of Flibanserin With HPLC-DAD-ESI-IT-TOF-MSn and Its Pharmaceutical Formulation Analysis With Inter-Laboratory Comparison.: In dieser Studie wird die Ascentis® Express Phenyl-Hexyl, 2,7 μm HPLC-Säule zur Charakterisierung von Abbauprodukten von Flibanserin verwendet, was ihre Wirksamkeit in hochauflösenden analytischen Anwendungen belegt (Graven et al., 2023).
  • HPLC with spectrophotometric or mass spectrometric detection for quantifying very-long chain fatty acids in human plasma and its association with cardiac risk factors.: Die Ascentis®-Express-Phenyl-Hexy-Säule wurde für die präzise Quantifizierung von Fettsäuren eingesetzt und verbindet ihre Verwendung mit der kritischen klinischen Forschung zu kardialen Risikofaktoren (Sharetha et al., 2021).
  • A fully automated and fast method using direct sample injection combined with fused-core column on-line SPE-HPLC for determination of ochratoxin A and citrinin in lager beers.: Diese Studie unterstreicht die Effizienz der Ascentis®-Express-Phenyl-Hexy-Säule beim Nachweis von Verunreinigungen in Getränken sowie ihre Rolle bei der Analyse der Lebensmittelsicherheit (Lhotska et al., 2016).
  • A study of retention characteristics and quality control of nutraceuticals containing resveratrol and polydatin using fused-core column chromatography.: In dieser Studie werden die Retentionseigenschaften von Nahrungsergänzungsmitteln unter Verwendung der Ascentis®-Express-Phenyl-Hexy-Säule untersucht und der Nutzen des Systems bei der Qualitätskontrolle von Nahrungsergänzungsmitteln aufgezeigt (Fibigr et al., 2016).
  • An on-line SPE-HPLC method for effective sample preconcentration and determination of fenoxycarb and cis, trans-permethrin in surface waters.: Die Ascentis®-Express-Phenylhexyl-Säule war ein wesentlicher Bestandteil bei der Vorkonzentration und Analyse von Pestizidrückständen in Umweltproben, was ihre Anwendungen für Umweltanalysen unterstreicht (Satinsky et al., 2015).

Rechtliche Hinweise

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
USXV004655
USXV004654
USXV004653
USXV004652
USXV004651

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

C M Chavez-Eng et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1011, 204-214 (2016-01-17)
An ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous determination of (4S,5R)-5-[3,5-bis (trifluoromethyl)phenyl]-3-{[4'-fluoro-5'-isopropyl-2'-methoxy-4-(trifluoromethyl)biphenyl-2-yl] methyl}-4-methyl-1,3-oxazolidin-2-one (anacetrapib, I) and [(13)C5(15)N]-anacetrapib, II in human plasma has been developed to support a clinical study to determine the absolute bioavailability of I.
E Lesellier
Journal of chromatography. A, 1266, 34-42 (2012-11-03)
The recent introduction of new stationary phases for liquid chromatography based on superficially porous particles, called core-shell or fused-core, dramatically improved the separation performances through very high efficiency, due mainly to reduced eddy diffusion. However, few studies have evaluated the
Petr Chocholouš et al.
Talanta, 103, 221-227 (2012-12-04)
Currently, for Sequential Injection Chromatography (SIC), only reversed phase C18 columns have been used for chromatographic separations. This article presents the first use of three different stationary phases: three core-shell particle-packed reversed phase columns in flow systems. The aim of
Ivona Lhotská et al.
Analytical and bioanalytical chemistry, 408(12), 3319-3329 (2016-03-20)
A new fast and sensitive method based on on-line solid-phase extraction on a fused-core precolumn coupled to liquid chromatography with fluorescence detection has been developed for ochratoxin A (OTA) and citrinin (CIT) determination in lager beer samples. Direct injection of
Alex D Batista et al.
Talanta, 133, 142-149 (2014-12-02)
On-line sample pretreatment (clean-up and analyte preconcentration) is for the first time coupled to sequential injection chromatography. The approach combines anion-exchange solid-phase extraction and the highly effective pentafluorophenylpropyl (F5) fused-core particle column for separation of eight sulfonamide antibiotics with similar
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Artikel

Ascentis® Express Phenyl Hexyl Columns for U/HPLC

Improve your reversed phase selectivity for polar aromatics and heterocyclic compounds with the Ascentis® Express Phenyl-Hexyl U/HPLC column.

Questions

1–10 of 10 Questions  

  1. Can a 100% aqueous mobile phase be used with the Phenyl-Hexyl HPLC column?

    1 answer

    1. The phenyl-hexyl phase is 100% aqueous compatible.

      Helpful?

  2. Can I use Ascentis Express on any type of HPLC system?

    1 answer

    1. Ascentis Express HPLC columns are capable of use on standard HPLC systems as well as UHPLC systems.  Columns are packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.

      Helpful?

  3. Can I use Ascentis Express on a UHPLC system?

    1 answer

    1. Yes.  Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments.  In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure.  These benefits include the capability of providing fast HPLC and higher resolution chromatography.  The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.  A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.

      Helpful?

  4. How can I measure my instrument bandwidth (IBW) and determine what Ascentis® Express HPLC Columns can be used with minimal efficiency loss created by too much internal instrument volume?

    1 answer

    1. The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.

      Helpful?

  5. What flow rate should I use with Ascentis® Express HPLC Columns?

    1 answer

    1. Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize Ascentis Express column efficiency. The suggested starting point for flow rate for Ascentis Express columns: 1.6 mL/min for 4.6 mm ID;  0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.

      Helpful?

  6. Can Ascentis® Express HPLC Columns be used for LC-MS?

    1 answer

    1. Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of Ascentis Express columns for high throughput UHPLC and LC-MS is that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.

      Helpful?

  7. Is there anything special I need to do to my HPLC system to use Ascentis Express?

    1 answer

    1. Nothing special is required to use Ascentis Express HPLC columns. To obtain the full benefits of Ascentis Express, one should minimize dispersion or instrument bandwidth in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level. For more information, request Guidelines for Optimizing Systems for Ascentis Express Columns (T407102)

      Helpful?

  8. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  9. What is the pressure rating for Ascentis® Express Phenyl-Hexyl HPLC Columns?

    1 answer

    1. This Ascentis Express column is stable to operating pressures up to 600 bar (9000 psi).

      Helpful?

  10. Do I need special fittings and tubing to connect Ascentis® Express HPLC Columns?

    1 answer

    1. While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.

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