The concentration of BSE in this product is approximately 100 mg/mL in DPBS. The lot-specific concentration is reported in the Certificate of Analysis as "Protein by Biuret". Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/o3008#product-documentation
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O3008
Oleinsäure-Albumin aus Rinderserum
liquid, sterile-filtered, BioReagent, suitable for cell culture
Oleic Acid-Albumin from bovine serum
Synonym(e):
Oleinsäure-BSA-Komplex
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
5 mL | Warenkorb auf Verfügbarkeit prüfen | € 56,30 |
Über diesen Artikel
Biological source:
bovine
Form:
liquid
Technique(s):
cell culture | mammalian: suitable
Impurities:
endotoxin, tested
Concentration:
100 mg/mL BSA in DPBS, 90-120 mg/mL protein (biuret)
Technischer Dienst
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Unterstützung erhaltenbiological source
bovine
Quality Segment
sterility
sterile-filtered
product line
BioReagent
form
liquid
composition
Oleic acid, 2 mol/mol albumin
concentration
100 mg/mL BSA in DPBS, 90-120 mg/mL protein (biuret)
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
storage temp.
2-8°C
Application
Ölsäure-Albumin aus Rinderserum wird für Folgendes verwendet: als Komponente von Dulbeccos Modified Eagle Medium (DMEM) zur Untersuchung der adipogenen Differenzierung in Fibroblastenzelllinien von Hühnerembryos[1], zur Untersuchung seiner Wirkung auf gutartige Prostataepithelzellen[2] als Komponente des Zellkulturmediums zur Quantifizierung von Triglyceriden in humanen Darmadenokarzinomepithelzellen[3]
Biochem/physiol Actions
Fettsäuren-Albumin-Komplexe werden bei der Entwicklung von serumfreien Medien als alternative Lipidquellen eingesetzt. Fettsäuren binden in starkem Maß an Serumproteine. Serumproteine können die Freisetzung von nützlichen Fettsäuren unterstützen und hemmende Fettsäuren binden. An Rinderserumalbumin (BSA) gebundene Ölsäure hat eine positive Wirkung auf das Wachstum verschiedener Zellen.
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Dieser Artikel | |||
|---|---|---|---|
| biological source bovine | biological source - | biological source bovine | biological source bovine |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| Quality Level 300 | Quality Level 300 | Quality Level 300 | Quality Level 300 |
| form liquid | form liquid | form solution | form liquid |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| impurities endotoxin, tested | impurities ≤400 EU/mL endotoxin | impurities ≤0.05% fatty acid, ≤200 EU/mL endotoxin | impurities endotoxin, tested |
Lagerklasse
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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What is the bsa concentration in the solution?
1 answer-
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Is it suitable to store this reagent in -20C in aliquots? We received our order recently and the shipping box has a large label of "Do not Freeze" I was inquiring if there was a decrease in concentration if frozen?
1 answer-
To effectively preserve the integrity of this product, it should be stored at the recommended temperature and protected from light. The stability and optimal performance may be impacted by the freeze/thaw process. This product should be stored at 2 to 8 C.
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How is the binding of oleic acid to BSA is confirmed?
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Many fatty acids have a natural affinity to some plasma proteins. In this case BSA serves as a carrier or transporter of the insoluble oleic acid. The product is tested for protein and oleic acid content, only. No additional testing is performed to confirm the complex formation. See the following publications for further information:
https://pubmed.ncbi.nlm.nih.gov/236351/
https://pubmed.ncbi.nlm.nih.gov/19745557/#:~:text=Albumin%20acts%20as%20main%20fatty,a%20several%20orders%20of%20magnitudeHelpful?
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What is the concentration of Oleic Acid in this product in Molarity (M) or milimolarity (mM)? I need this to be able to follow a protocol
1 answer-
Oleic acid has a molecular mass of 282.47 g/mol. In order to find the concentration of oleic acid in a specific lot, use the Oleic acid concentration on the lot-specific certificate of analysis (COA).
The equation used to find the concentration is as follows: (molar mass of Oleic acid) x (concentration of Oleic acid from COA), this will result in units of liter/mole which can be multiplied by the reciprocal, resulting in units of Molarity (mole/liter).
For example, for lot number SLCM9550, the Oleic acid concentration is reported as 940 ug/mL.
282.47 g/mol / 0.94 g/L =300.5 L/mol
1/300.5= 0.003327 M (mol/liter) = 3.33 mM Oleic AcidHelpful?
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Through what method is oleic acid complexed to BSA?
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Unfortunately, the method of preparation considered proprietary.
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