H8162

HIS-Select^® Kobalt-Affinitätsgel

(1:1 suspension in a 30% ethanol solution)

• Synonym(e): cobalt affinity gel, cobalt charged agarose
• UNSPSC-Code: 41106500
• NACRES: NA.56

Eigenschaften

• Form: (1:1 suspension in a 30% ethanol solution)
• Haltbarkeit: 1 yr (when stored properly.)
• Methode(n): protein purification: suitable
• Matrix: 6% Beaded Agarose
• Kapazität: >15 mg/mL, agarose binding capacity (protein)(with an approx. 30 kDa protein)
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

The HIS-Select Cobalt Affinity Gel is a quadridentate chelate on beaded agarose that is charged with cobalt. The gel is designed to specifically bind histidine-containing proteins / His-tag proteins. It is selective for recombinant proteins with histidine tags and exhibits low non-specific binding of other proteins.
HIS-Select Cobalt Affinity Gel is an immobilized metal-ion affinity chromatography (IMAC) product. The matrix for this affinity gel is 6% beaded agarose. The selectivity can be modulated with the inclusion of imidazole during chromatography. HIS-Select Cobalt Affinity Gel is durable and can capture recombinant proteins with histidine tags at a high flow rate. Recombinant proteins with His tags are bound using native, denaturing, or mild reducing conditions. The capacity of this affinity gel is typically >15 mg/mL of packed gel, determined with a His-tagged protein of molecular mass ~30 kDa.

Anwendung

HIS-Select Cobalt Affinity Gel allows for high purity, low non-specific binding, and high binding capacity of protein. HIS-Select Cobalt Affinity Gel also works well for purification of histidine-tagged proteins / His-tag proteins in native, denaturing, or mild reducing conditions. HIS-Select^® Cobalt Affinity Gel has been used to purify His-tagged proteins.[1]

Physikalische Form

1:1 suspension in a 30% ethanol solution

Rechtliche Hinweise

HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Piktogramme: GHS02,GHS08,GHS09
• Signalwort: Danger
• H-Sätze: H226,H317,H319,H334,H341,H350,H360F,H410
• P-Sätze: P210 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338 - P308 + P313
• Gefahreneinstufungen: Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Irrit. 2 - Flam. Liq. 3 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Sens. 1
• WGK: WGK 3
• Persönliche Schutzausrüstung: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter