G3907

Glutathion−-Agarose

set of 3 pre-packed columns (2.5 ml each), (1:1 suspension in a 0.5 M NaCl + 20% ethanol solution)

• Synonym(e): GSH-Agarose, S-verknüpfte Glutathion-Agarose
• MDL-Nummer: MFCD00131200
• UNSPSC-Code: 41106500
• NACRES: NA.56

Eigenschaften

• Qualitätsniveau: 200
• Form: (1:1 suspension in a 0.5 M NaCl + 20% ethanol solution)
• chemische Klasse(n) des Analyten: proteins (GST)
• Verpackung: set of 3 pre-packed columns (2.5 ml each)
• Methode(n): immunoprecipitation (IP): suitable; protein purification: suitable
• Matrix: cross-linked 4% beaded agarose
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

The resin in these columns consists of glutathione attached through the sulfur to epoxy activated, 4% cross-linked beaded agarose resulting in a 12 atom spacer. [1]

Anwendung

Affinity chromatography using glutathione-agarose permits rapid, mild, non-denaturing and highly selective purification of glutathione binding enzymes such as glutathione-S-transferase, glutathione peroxidase, and glyoxalase I.[2]
The product was used in the design, synthesis and biological evaluation of new tryptamine and tetrahydro-β-carboline-based selective inhibitors of CDK4.[3] It was also used to study Fascaplysin-inspired diindolyls as selective inhibitors of CDK4/cyclin D1.[4]

Die Affinitätschromatographie unter Verwendung von Glutathion-Agarose ermöglicht eine schnelle, milde, nicht denaturierende und hochselektive Aufreinigung von Proteinen, die Glutathion bindende Sequenzen, wie Glutathion-S-Transferase (GST), Glutathion-Peroxidase und Glyoxalase I, enthalten.

Verlinkung

Suspension of Product No. G 4510

Physikalische Form

1:1 suspension in a 0.5 M NaCl + 20% ethanol solutionl

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): 180.1 °F
• Flammpunkt (°C): 82.3 °C