The K12 strain information can be found on the UniProt website under the following entry: P0A9C5.
Alle Fotos(1)
Wichtige Dokumente
G1270
L-Glutamine Synthetase from Escherichia coli
lyophilized powder, 400-2,000 units/mg protein
Synonym(e):
L-Glutamate:ammonia ligase (ADP-forming)
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Größe auswählen
100 UNITS
€ 544,00
Alle Fotos(1)
Größe auswählen
Ansicht ändern
100 UNITS
€ 544,00
About This Item
CAS-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352204
NACRES:
NA.26
Empfohlene Produkte
Form
lyophilized powder
Qualitätsniveau
Spezifische Aktivität
400-2,000 units/mg protein
Aufgereinigt durch
affinity chromatography
Enthält
dithioerythritol as preservative
Zusammensetzung
Protein, ~5% Lowry
Löslichkeit
H2O: soluble 0.95-1.05 mg/mL, clear to hazy
UniProt-Hinterlegungsnummer
Fremdaktivität
ATPase <0.2%
Lagertemp.
−20°C
Angaben zum Gen
Escherichia coli K12 ... glnA(948370)
Allgemeine Beschreibung
L-Glutamine Synthetase from bacteria shows dodecameric structure comprising of 12 active sites. Each active site termed bifunnel, has an ATP and glutamate binding sites. The dodecamer is stabilized by two hexameric rings.[1]
Anwendung
L-Glutamine Synthetase from Escherichia coli has been used in the synthesis of methylglutamine from methylammonium in E coli[2] and in the glutamine synthetase protection activity of human thioredoxin peroxidase enzyme, AOE372.[3]
L-Glutamine synthetase may be used for the purification of proteases from Escherichia coli.[4]
Biochem./physiol. Wirkung
L-glutamine synthetase catalyzes the condensation of L-glutamate and ammonia to L-glutamine[5]. It is a degradative enzyme for glutamic acid.
Nitrogen starvation dictates the expression of the glutamine synthetase (GS) gene in E. coli.[1] GS plays a key role in ammonia assimilation in bacteria.[6] Adenylylation of GS is catalyzed by adenylyltransferase. Adenylylation of GS modulates its catalytic functionality resulting in glutamine limitation in E coli.[7]
Degradative enzyme for glutamic acid
Einheitendefinition
One unit will convert 1.0 μmole of L-glutamate to L-glutamine in 15 min at pH 7.1 at 37 °C.
Physikalische Form
Contains potassium phosphate, sodium citrate and magnesium acetate buffer salts
Signalwort
Danger
H-Sätze
P-Sätze
Gefahreneinstufungen
Resp. Sens. 1
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
Hier finden Sie alle aktuellen Versionen:
Analysenzertifikate (COA)
Lot/Batch Number
Die passende Version wird nicht angezeigt?
Wenn Sie eine bestimmte Version benötigen, können Sie anhand der Lot- oder Chargennummer nach einem spezifischen Zertifikat suchen.
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Kunden haben sich ebenfalls angesehen
Regulatory role for a novel human thioredoxin peroxidase in NF-kappaB activation
Jin DY, et al.
The Journal of Biological Chemistry, 272(49), 30952-30961 (1997)
Reversible Adenylylation of Glutamine Synthetase Is Dynamically Counterbalanced during Steady-State
Okano H, et al.
Journal of molecular biology, 404(1), 522-536 (2010)
Huijuan Jia et al.
Molecular nutrition & food research, 57(2), 291-306 (2012-11-21)
This study addresses the effects of branched-chain amino acids (BCAA) on global gene expression in liver and skeletal muscle and the molecular mechanisms underlying the improvement in liver cirrhosis using DNA microarray analysis combined with RNase protection assay. Male Wistar
Peng Jiang et al.
Biochemistry, 51(45), 9032-9044 (2012-10-24)
Uridylyltransferase/uridylyl-removing enzyme (UTase/UR) catalyzes uridylylation of PII and deuridylylation of PII-UMP, with both activities regulated by glutamine. In a reconstituted UTase/UR-PII cycle containing wild-type UTase/UR, the steady-state modification of PII varied from nearly complete modification to nearly complete demodification as
Structural Analysis of Glutamine Synthetase from Helicobacter pylori
Joo HK, et al.
Scientific Reports, 8(1), 11657-11657 (2018)
-
From which E. coli strain is your product G1270 L-Glutamine Synthetase from Escherichia coli isolated?
1 answer-
Helpful?
-
Active Filters
Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..
Setzen Sie sich mit dem technischen Dienst in Verbindung.
