G4509

Glycerokinase aus E. coli

lyophilized powder, 40-100 units/mg protein

• Synonym(e): ATP:glycerol 3-phosphotransferase, Glycerin-Kinase
• CAS-Nummer: 9030-66-4
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-862-0
• MDL number: MFCD00066766
• EG-Nummer: 2.7.1.30 (BRENDA, IUBMB)
• eCl@ss: 32160410
• Specific activity: 40-100 units/mg protein
• Biological source: Escherichia coli

Eigenschaften

• biological source: Escherichia coli
• Quality Segment: 200
• form: lyophilized powder
• specific activity: 40-100 units/mg protein
• composition: Protein, ≥50% biuret
• foreign activity: Triose-phosphate isomerase, hexokinase, myokinase, glycerol dehydrogenase and NADH oxidase ≤0.05%
• storage temp.: −20°C

Beschreibung

Application

Glycerol kinase (glpK) was used to study the effects of pain controlling neuropeptides on human fat cell lipolysis.

Biochem/physiol Actions

Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. It is also subject to feedback regulation by fructose-1,6-bisphosphate.

Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. <<<20,21>>> It is also subject to feedback regulation by fructose-1,6-bisphosphate.<<<22>>>

Physical form

Teilweise gereinigtes lyophilisiertes Pulver, Rest ist vorwiegend Salze und EDTA

Other Notes

1 U setzt 1.0 μmol Glycerin und ATP zu L-α-Glycerophosphat und ADP pro Minute um bei pH 9.8 und 25°C in einem gekoppelten System mit PK/LDH.

Sicherheitshinweise

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)