Das Adenosin-5’-triphosphat (ATP) Biolumineszenz-Assaykit kann für die quantitative Bestimmung von Adenosin-5’-triphosphat (ATP) mittels Biolumineszenz in Proben mit 2 x 10–12 bis 8 x 10–5 Mol/Liter verwendet werden. Wenn die Luciferase die Oxidation von D-Luciferin katalysiert, wird ATP verbraucht und Licht emittiert.
Anwendung
Das ATP-Assay-Kit wurde für die Messung des Gehalts an zellulärem ATP in isolierten mitochondrialen Fraktionen aus L. donovani promastigotes verwendet. [1]
Der Adenosin-5′-triphosphat (ATP) Assay-Mix wurde auch für die Messung des Gehalts an zellulärem ATP im ATP-Lebensfähigkeits-Assay verwendet.[2][3][4]
Komponenten
Enthält Luciferase, Luciferin, MgSO4, EDTA, DTT, BSA und Tricin-Puffer.
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