This product can be reconstituted in ammonium hydroxide, pH >9, to a minimum of 1 mg/mL, or in DMSO at 0.4–4 mM. Stock solutions can then be serially diluted into aqueous buffers, such as PBS or saline, to the desired working concentrations. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/301/154/a9810pis.pdf
Fortfahren mit
A9810
Amyloid-β-Proteinfragment 1-42
Anmelden zur Ansicht der Organisations- und Vertragspreise.
Größe auswählen
Ansicht ändern
| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
0.1 mg | Warenkorb auf Verfügbarkeit prüfen | € 253,00 |
Über diesen Artikel
Empirische Formel (Hill-System):
C203H311N55O60S
CAS-Nummer:
Molekulargewicht:
4514.04
UNSPSC Code:
12352202
NACRES:
NA.32
MDL number:
Technischer Dienst
Benötigen Sie Hilfe? Unser Team von erfahrenen Wissenschaftlern ist für Sie da.
Unterstützung erhaltenassay
≥95% (HPLC)
Quality Segment
form
powder
UniProt accession no.
storage temp.
−20°C
SMILES string
S(CC[C@H](NC(=O)[C@@H](NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(
InChI
1S/C203H311N55O60S/c1-28-106(20)164(195(310)220-91-149(267)228-130(71-98(4)5)181(296)238-129(66-70-319-27)179(294)251-158(100(8)9)193(308)218-87-146(264)215-88-151(269)250-160(102(12)13)198(313)255-163(105(18)19)199(314)258-165(107(21)29-2)200(315)227-112
InChI key
DZHSAHHDTRWUTF-SIQRNXPUSA-N
Gene Information
human ... APP(351)
General description
Amyloid-β-Protein wird aus dem Amyloid-β-Präkursor-Protein (APP) gebildet. Es besteht aus zwei C-terminalen Varianten, z. B. Aβ 1-42 mit langer Kette und Aβ 1-40 mit kurzer Kette. APP ist auf dem menschlichen Chromosom 21q21.3 lokalisiert.
Application
Amyloid-β-Proteinfragment 1-42 wird wie folgt verwendet:
- zur Herstellung von Aβ-1-42-Oligomer[1]
- in Western-Blot-Analyse
- zum Störungstest von immunomagnetischem Reduktions(IMR)-Plasma-Aβ42-Assay
- zur Untersuchung der Wirkung von Resveratrol auf Aβ-1-42-induzierte Beeinträchtigung von räumlichem Lernen, Erinnerung und synaptischer Plastizität
- zur Untersuchung der Wirkung von Aβ in Epithelzellkulturen
Biochem/physiol Actions
Das am häufigsten vorkommende Fragment von Amyloid-β-Protein im Gehirn von Patienten mit Alzheimer′-Krankheit und Down′-Syndrom.
Amyloid-β-Proteinfragment 1-42 (Aβ 1-42) weist antioxidative und neuroprotektive Eigenschaften auf. Eine Ansammlung von Amyloid-β-Protein wird mit Alzheimer′-Krankheit (AD) und Down-Syndrom in Verbindung gebracht. Aβ 1-42 reguliert den Cholesterintransport und kann als Transkriptionsfaktor agieren. Es kann entzündungshemmende und antimikrobielle Eigenschaften aufweisen.
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| assay ≥95% (HPLC) | assay ≥95% (HPLC) | assay ≥90% (HPLC) | assay ≥97% (HPLC) |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form powder | form powder | form powder | form powder |
| Gene Information human ... APP(351) | Gene Information human ... APP(351) | Gene Information human ... APP(351) | Gene Information human ... APP(351) |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Hier finden Sie alle aktuellen Versionen:
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
-
What would be the best method to reconstitute this amyloid β protein fragment 1-42 into the concentrations starting at 0.1nM and working my way up to 5, 10 and 100nM?
1 answer-
Helpful?
-
-
After dissolving in DMSO, I want to dilute it with saline (finally 1ml/1mg) and use it for i.c.v. injection in mice. How many µl of 0.4-4 mM DMSO per 0.1 mg should I dissolve in?
1 answer-
This product is soluble in DMSO at 0.4 - 4 mM, as stated in the product datasheet. This equates to 1.8 - 18 mg/mL in DMSO. This stock solution may be diluted with aqueous buffers, such as PBS or saline, to the desired working concentration. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/301/154/a9810pis.pdfHelpful?
-
-
What is the difference between the AB peptide (1-42)(PP69) and AB protein fragment (1-42)(A9810)?
1 answer-
These products are functionally the same, but are different brands. The PP69 is a Calbiochem product, while the A9810 is from Sigma-Aldrich. The testing specifications are slightly different, however both offer a minimum purity of 95%. The A9810 is lyophilized from a solution of water and TFA. The PP69 is lyophilized from a solution of 50 mM Tris-HCl. Please see the links below to review the product datasheet for each:
PP69 Calbiochem:
https://www.emdmillipore.com/product/-Amyloid-Peptide-1-42-Human,EMD_BIO-PP69#anchor_PDS
A9810 Sigma-Aldrich:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/301/154/a9810pis.pdfHelpful?
-
-
It is instructed to dissolve the product in Ammonium Hydroxide solution, but what should be the molarity / Concentration of the ammonium hydroxide solution
1 answer-
This product can be solubilized in ammonium hydroxide at a pH greater than 9. The concentration is not reported. It is recommended to prepare at a minimum 1 mg/mL concentration.
Helpful?
-
-
How much ammonium hydroxide (pH >9) should be added to the 0,1 mg vial ? Is it possible to vortex the vial ?
1 answer-
It is recommended to prepare a solution at a minimum of 1 mg/mL of ammonium hydroxide. Vertexing the vial is not necessary.
Helpful?
-
-
in which solvent it should be dissolved ?
1 answer-
As per the product information sheet, this product is soluble in ammonium hydroxide, pH >9, or in DMSO at 0.4–4 mM. Solutions can then be diluted into aqueous buffers such as10 mM sodium phosphate for use. Other reported solvents include hexafluoroisopropanol, acetonitrile, and acetic acid.
See the link below to review the product information sheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/301/154/a9810pis.pdfHelpful?
-
-
What is the pI of Product A9810, Amyloid β Protein Fragment 1-42?
1 answer-
The pI as calculated from the amino acid sequence for Product A9810, Amyloid β Protein Fragment 1-42, is 5.31.
Helpful?
-
-
What is the source of Product A9810, Amyloid β Protein Fragment 1-42?
1 answer-
This protein fragment is a synthetic organic. The gene for this peptide is located on human chromosome 21.
Helpful?
-
-
Is the mouse protein fragment the same as the human protein fragment in Product A9810, Amyloid β Protein Fragment 1-42?
1 answer-
The homology is 93% by identity, 95% positives... no gaps
Helpful?
-
-
What is the secondary structure of Product A9810, Amyloid β Protein Fragment 1-42?
1 answer-
The secondary structure of this fragment will exist as a mixture of B-sheet, a-helix, and random coil structures dependent upon the solution conditions as described in J. Mol. Biol., 225, 1075-1083, 1992
Helpful?
-