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A9810

Sigma-Aldrich

Amyloid-β-Proteinfragment 1-42

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About This Item

Empirische Formel (Hill-System):
C203H311N55O60S
CAS-Nummer:
107761-42-2
Molekulargewicht:
4514.04
MDL-Nummer:
MFCD00163049
UNSPSC-Code:
12352202
NACRES:
NA.32

€ 243,00

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Assay

≥95% (HPLC)

Qualitätsniveau

200

Form

powder

UniProt-Hinterlegungsnummer

P05067

Lagertemp.

−20°C

SMILES String

S(CC[C@H](NC(=O)[C@@H](NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(

InChI

1S/C203H311N55O60S/c1-28-106(20)164(195(310)220-91-149(267)228-130(71-98(4)5)181(296)238-129(66-70-319-27)179(294)251-158(100(8)9)193(308)218-87-146(264)215-88-151(269)250-160(102(12)13)198(313)255-163(105(18)19)199(314)258-165(107(21)29-2)200(315)227-112

InChIKey

DZHSAHHDTRWUTF-SIQRNXPUSA-N

Angaben zum Gen

human ... APP(351)

Amino Acid Sequence

Asp-Ala-Glu-Phe-Arg-His-Asp-Ser-Gly-Tyr-Glu-Val-His-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Glu-Asp-Val-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-Val-Gly-Gly-Val-Val-Ile-Ala

Allgemeine Beschreibung

Amyloid-β-Protein wird aus dem Amyloid-β-Präkursor-Protein (APP) gebildet. Es besteht aus zwei C-terminalen Varianten, z. B. Aβ 1-42 mit langer Kette und Aβ 1-40 mit kurzer Kette.[1] APP ist auf dem menschlichen Chromosom 21q21.3 lokalisiert.[2]

Anwendung

Amyloid-β-Proteinfragment 1-42 wird wie folgt verwendet:
  • zur Herstellung von Aβ-1-42-Oligomer[3][4]
  • in Western-Blot-Analyse[5]
  • zum Störungstest von immunomagnetischem Reduktions(IMR)-Plasma-Aβ42-Assay[6]
  • zur Untersuchung der Wirkung von Resveratrol auf Aβ-1-42-induzierte Beeinträchtigung von räumlichem Lernen, Erinnerung und synaptischer Plastizität[7]
  • zur Untersuchung der Wirkung von Aβ in Epithelzellkulturen[1]

Biochem./physiol. Wirkung

Amyloid-β-Proteinfragment 1-42 (Aβ 1-42) weist antioxidative und neuroprotektive Eigenschaften auf.[8] Eine Ansammlung von Amyloid-β-Protein wird mit Alzheimer′-Krankheit (AD) und Down-Syndrom in Verbindung gebracht.[9] Aβ 1-42 reguliert den Cholesterintransport[10] und kann als Transkriptionsfaktor agieren.[11] Es kann entzündungshemmende und antimikrobielle Eigenschaften aufweisen.[12]
Das am häufigsten vorkommende Fragment von Amyloid-β-Protein im Gehirn von Patienten mit Alzheimer′-Krankheit und Down′-Syndrom.

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Flammpunkt (°F)

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Flammpunkt (°C)

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Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
0000388520
0000387190
0000368432
0000336675
0000331858

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Resveratrol ameliorates spatial learning memory impairment induced by Abeta1-42 in rats
Wang R, et al.
Neuroscience, 344, 39-47 (2017)
Kyunghee Byun et al.
PloS one, 7(5), e37917-e37917 (2012-06-05)
Advanced glycation end products (AGEs) have long been considered as potent molecules promoting neuronal cell death and contributing to neurodegenerative disorders such as Alzheimer's disease (AD). In this study, we demonstrate that AGE-albumin, the most abundant AGE product in human
Function of beta-amyloid in cholesterol transport: a lead to neurotoxicity
Yao ZX and Papadopoulos V
Faseb Journal, 16(12), 1677-1679 (2002)
Magnesium Ions Inhibit the Expression of Tumor Necrosis Factor alpha and the Activity of gamma-secretase in a beta-amyloid Protein-dependent Mechanism in APP/PS1 Transgenic Mice
Yu X, et al.
Frontiers in Molecular Neuroscience, 11 (2018)
Plasma Amyloid-beta(Abeta 42) Correlates with Cerebrospinal Fluid Abeta 42 in Alzheimer?s Disease
Teunissen CE, et al.
Journal of Alzheimer'S Disease, 1-7 (2018)
Alle zugehörigen Dokumente anzeigen

Questions

1–10 of 10 Questions  

  1. After dissolving in DMSO, I want to dilute it with saline (finally 1ml/1mg) and use it for i.c.v. injection in mice. How many µl of 0.4-4 mM DMSO per 0.1 mg should I dissolve in?

    1 answer

    1. This product is soluble in DMSO at 0.4 - 4 mM, as stated in the product datasheet. This equates to 1.8 - 18 mg/mL in DMSO. This stock solution may be diluted with aqueous buffers, such as PBS or saline, to the desired working concentration. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/301/154/a9810pis.pdf

      Helpful?

  2. What is the difference between the AB peptide (1-42)(PP69) and AB protein fragment (1-42)(A9810)?

    1 answer

    1. These products are functionally the same, but are different brands. The PP69 is a Calbiochem product, while the A9810 is from Sigma-Aldrich. The testing specifications are slightly different, however both offer a minimum purity of 95%. The A9810 is lyophilized from a solution of water and TFA. The PP69 is lyophilized from a solution of 50 mM Tris-HCl. Please see the links below to review the product datasheet for each:
      PP69 Calbiochem:
      https://www.emdmillipore.com/product/-Amyloid-Peptide-1-42-Human,EMD_BIO-PP69#anchor_PDS
      A9810 Sigma-Aldrich:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/301/154/a9810pis.pdf

      Helpful?

  3. It is instructed to dissolve the product in Ammonium Hydroxide solution, but what should be the molarity / Concentration of the ammonium hydroxide solution

    1 answer

    1. This product can be solubilized in ammonium hydroxide at a pH greater than 9. The concentration is not reported. It is recommended to prepare at a minimum 1 mg/mL concentration.

      Helpful?

  4. How much ammonium hydroxide (pH >9) should be added to the 0,1 mg vial ? Is it possible to vortex the vial ?

    1 answer

    1. It is recommended to prepare a solution at a minimum of 1 mg/mL of ammonium hydroxide. Vertexing the vial is not necessary.

      Helpful?

  5. in which solvent it should be dissolved ?

    1 answer

    1. As per the product information sheet, this product is soluble in ammonium hydroxide, pH >9, or in DMSO at 0.4–4 mM. Solutions can then be diluted into aqueous buffers such as10 mM sodium phosphate for use. Other reported solvents include hexafluoroisopropanol, acetonitrile, and acetic acid.

      See the link below to review the product information sheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/301/154/a9810pis.pdf

      Helpful?

  6. What is the secondary structure of Product A9810, Amyloid β Protein Fragment 1-42?

    1 answer

    1. The secondary structure of this fragment will exist as a mixture of B-sheet, a-helix, and random coil structures dependent upon the solution conditions as described in J. Mol. Biol., 225, 1075-1083, 1992

      Helpful?

  7. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  8. What is the source of Product A9810, Amyloid β Protein Fragment 1-42?

    1 answer

    1. This protein fragment is a synthetic organic.  The gene for this peptide is located on human chromosome 21.

      Helpful?

  9. What is the pI of Product A9810, Amyloid β Protein Fragment 1-42?

    1 answer

    1. The pI as calculated from the amino acid sequence for Product A9810, Amyloid β Protein Fragment 1-42, is 5.31.

      Helpful?

  10. Is the mouse protein fragment the same as the human protein fragment in Product A9810, Amyloid β Protein Fragment 1-42?

    1 answer

    1. The homology is 93% by identity, 95% positives...  no gaps

      Helpful?

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