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Wichtige Dokumente
83702
Rhodamin 123
BioReagent, for fluorescence, ≥85% (HPLC)
Synonym(e):
2-(6-Amino-3-imino-3H-xanthen-9-yl)-benzoesäure-methylester
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About This Item
Lineare Formel:
C21H17ClN2O3
CAS-Nummer:
Molekulargewicht:
380.82
Beilstein:
6030951
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352116
PubChem Substanz-ID:
NACRES:
NA.32
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Empfohlene Produkte
Qualität
for fluorescence
Produktlinie
BioReagent
Assay
≥85% (HPLC)
Form
powder
Verunreinigungen
≤10% water
Löslichkeit
ethanol: 20 mg/mL, clear, red
Eignung
in accordance for fluorescence
suitable for fluorescence
SMILES String
Cl[H].COC(=O)c1ccccc1C2=C3C=CC(=N)C=C3Oc4cc(N)ccc24
InChI
1S/C21H16N2O3.ClH/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20;/h2-11,22H,23H2,1H3;1H
InChIKey
MYFATKRONKHHQL-UHFFFAOYSA-N
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Verwandte Kategorien
Allgemeine Beschreibung
Rhodamine 123 is a fluorescent dye.[1]
Anwendung
Rhodamine 123 has been used:
- as a substrate for P-glycoprotein (P-gp) to read out functional transporter activity in fluorescent accumulation assays[2]
- to label the cells for estimating the P-gp expression by flow cytometry[1]
- in transport studies, was added to the apical to basolateral (AB-transport) or basolateral to apical (BA-transport)[3]
- the detection of mitochondrial membrane potential (ΔΨm)[4]
Biochem./physiol. Wirkung
Fluorescent dye most commonly used in flow cytometry as functional reporter for Pgp. Functional assays for MDR proteins are better prognostic indicators in cancer therapy than levels of MDR protein expression. Rh 123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA (7-amino-4-methylcoumarin-3-acetic acid). Recent reports indicate Rh 123 may also be a substrate of MRP1.
Rhodamine 123 is usually used as a functional reporter for P-glycoprotein (P-gp) in flow cytometry.[1]
Sonstige Hinweise
Supravital mitochondrial-specific dye[5]
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
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The use of the supravital mitochondrial-specific dye Rhodamine 123 (Rh 123) in combination with flow cytometry permits the monitoring of the changes in the mitochondrial transmembrane potential, reflecting the overall mitochondrial activity of the living cell. While this probe appears
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Which is the storage conditions recommend for this product?
1 answer-
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What are the excitation and emission wavelengths for Rhodamine 123 (Product Nos. 83702 and R8004)?
1 answer-
Using ethanol as the solvent, the excitation maximum is 511 nm and the emission maximum is 534 nm. The recommended excitation and emission wavelengths in PBS are 488 and 515-575 nm, respectively.
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Why would one select Rhodamine 123 (Product Nos. 83702 and R8004) instead of other rhodamine compounds?
1 answer-
Due to is comparatively low toxicity, Rhodamine 123 is the dye of choice for visualizing mitochondria or for cell viability measurements.
Helpful?
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What is the solubility of Rhodamine 123, (Product Nos. 83702 and R8004)?
1 answer-
Rhodamine 123 is soluble in ethanol at 20 mg/mL, and has been reported soluble in ether, various fats and lipids, and has limited solubility in water (no concentration given).
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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