The solution stability of this product has not been determined. However, other collagenase products available have been determined to be stable when stored in solution at –20°C. Repeated freeze/thaw cycles are not recommended and may result in a decline in activity. Please see the link below to review the product datasheet for C2139, a collagenase preparation for the isolation of adipocytes and hepatocytes:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/173/127/c2139pis.pdf
C2-BIOC
Kollagenase Typ II, Cls II
Synonym(e):
Collagen A
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100 MG
€ 48,30
1 G
€ 300,00
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100 MG
€ 48,30
1 G
€ 300,00
About This Item
UNSPSC-Code:
12352204
NACRES:
NA.78
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Allgemeine Beschreibung
Dieses Produkt ist identisch mit Biochrom GmbH Artikelnummern C2-22 und C2-28. Enzymgemisch aus Kollagenase und Clostripain mit tryptischer und proteolytischer Aktivität aus Clostridium histolyticum. Diese Zubereitung weist eine hohe Clostripain-Aktivität auf, und die tryptische Aktivität ist nahe an Typ I. Geeignet für Gewebe aus Leber, Knochen, Schilddrüse, Herz und Speicheldrüse. Die spezifische Aktivität beträgt 125 bis 250 Mandl-Einheiten je Milligramm pulverförmige Substanz.
Kollagenase Typ II (Klasse II) ist eines der Haupt-Gewebedissoziationsenzyme, die von Clostridium histolyticum erzeugt werden. Sie unterstützt den Verdau der von der Kollagenase Typ I, erzeugten kleineren Kollagenfragmente. Kollagenasen, die der Familie der Matrix-Metalloproteasen (MMP) angehören, verdauen Kollagen in der extrazellulären Matrix (ECM). Kollagene sind Proteine mit Dreifachhelix-Struktur, die drei Kollagen-Polypeptide mit hoher Zugfestigkeit umfassen.[1]
Anwendung
Kollagenase Typ II (Klasse II) wird wie folgt verwendet:
- als Komponente von Roswell Park Memorial Institute(RPMI)-1640-Medien für den Verdau von Lebergewebe[2]
- zum Perfundieren von Mäuseherzen für die Isolierung von epikardialen Stromazellen (EpiSC)[3]
- für Verdau und Injektion der Kollagenase in die intrarenale Aorta abdominalis zur Nierenperfusion.[4]
Hier finden Sie alle aktuellen Versionen:
Analysenzertifikate (COA)
Lot/Batch Number
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Dear Sir, I stored reconstituted collagenase in a -20 degrees fridge after diluting and using some of it, after 4months is the enzyme still viable for use? It was left in -20 degrees all the while, thanks
1 answer-
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Kann die Kollagenase für die enzymatische Verdauung und Isolierung von Hühnerembryoosteoblasten verwendet werden? Wie ist die Reinheit des Produkts?
1 answer-
This product has not been tested for use in isolating osteoblasts from chick embryos. Currently, there is no collagenase product option that has been tested for suitability in this application. The product is generally used for bone tissue digests. However the end user would have to determine suitability experimentally.
A preliminary search of PubMed and Google Scholar did not find research articles citing this specific application. The working enzyme concentration range is typically 0.1 to 0.2% (w/v), depending on the delicate nature of the tissue substrate. This product is tested for enzymatic activity. The analytical purity has not been determined.
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골관절염 모델링에 있어 collagenase type 2 를 500 U로 Intra-articular injection 해야합니다만, spec sheet 에 쓰여있는 바로는 units/mg Solid (Collagen) 이 >= 125 로 되어있는데 mg 당 정확한 unit을 알수는 없나요 ?
1 answer-
The exact value is reported on the lot specific Certificate of Analysis. Please see the link below to access a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sial/c2bioc#product-documentationHelpful?
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Dear sir I was bought collagenase before, but I can't find my instruction manual. Can I have a copy? I need to prepare 0.2% solution to dissolve tumor cells, and I want to grow solute for greenhouse configuration and two for dissolving cells.
1 answer-
Unfortunately, no user manual is provided with this product. It is recommended to prepare working solution by dissolving the enzyme in water to a final concentration of 100mg/mL. Dilute with PBS (Ca2+/Mg2+-free) to a final concentration of 1mg/mL and filter through a sterile 0.2μm membrane filter.
Another Collagenase Type II product under the Sigma-Aldrich brand, C6885, does offer a product information sheet. While the lot-specific activity between the two products varies, the storage, handling, and functional information would be the same. See the link below to review this document:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/321/500/c6885pis.pdfHelpful?
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