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重要文件

P8849

Sigma-Aldrich

Protease Inhibitor Cocktail

DMSO solution, for the inhibition of serine, cysteine, aspartic, aminopeptidases and thermolysin-like activities, for use in purification of Histidine-tagged proteins, DMSO solution

同義詞:

Protease inhibitor solution

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About This Item

MDL號碼:
分類程式碼代碼:
12352200
NACRES:
NA.77
形狀:
DMSO solution
儲存溫度:
−20°C

產品名稱

Protease Inhibitor Cocktail, for use in purification of Histidine-tagged proteins, DMSO solution

生物源

synthetic

品質等級

形狀

DMSO solution

儲存溫度

−20°C

尋找類似的產品? 前往 產品比較指南

一般說明

蛋白酶抑制剂的DMSO溶液专用于纯化组氨酸标签蛋白。

溶液中混有多种抑制剂成分,可特异性抑制丝氨酸、半胱氨酸、天冬氨酸、嗜热菌蛋白酶样蛋白酶和氨基肽酶。

特異性

抑制丝氨酸,半胱氨酸,天冬氨酸,和嗜热菌蛋白酶样蛋白酶和氨基肽酶。

應用

混合液经过专门的优化和验证,适合分离组氨酸标签(His-tag)蛋白质,特意去除螯合剂后尤其适合用于固定化金属亲和层析(IMAC)分离。

推荐用量:1 mL溶液抑制20g大肠杆菌细胞或10g杆状病毒感染草地夜蛾蛹卵巢细胞得到的100ml细胞裂解液中的蛋白酶活性。

特點和優勢

专门为组氨酸标签蛋白纯化而配制。

靶向多种蛋白酶,具有全面的蛋白酶抑制活性。

不加螯合剂,适合IMAC应用。

方便的包装选择。

成分

AEBSF
乌苯美司
E-64
Pepstatin A
磷阿米酮

數量

推荐采用1mL抑制从20gEscherichia coli或10g杆状病毒感染 Spodoptera frugiperda蛹卵巢细胞提取的总体积100ml的蛋白酶。

该蛋白酶抑制剂混合物已针对组蛋白标记蛋白进行了优化和测试

準備報告

该产品在DMSO中是澄清溶液。 推荐1mL溶液可以抑制20gE. Coli细胞或10g细胞或10g 杆状病毒感染细胞得到的100ml细胞裂解液中的蛋白酶活性。

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

185.0 °F - closed cup

閃點(°C)

85 °C - closed cup


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分析證明 (COA)

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Z Kurowska et al.
Neuroscience, 256, 456-466 (2013-10-26)
Nogo-A is a transmembrane protein originally discovered in myelin, produced by postnatal CNS oligodendrocytes. Nogo-A induces growth cone collapse and inhibition of axonal growth in the injured adult CNS. In the intact CNS, Nogo-A functions as a negative regulator of
E Boudreau et al.
The EMBO journal, 19(13), 3366-3376 (2000-07-06)
The psbD mRNA, which encodes the D2 reaction center polypeptide of photosystem II, is one of the most abundant chloroplast mRNAs. We have used genomic complementation to isolate the nuclear Nac2 gene, which is required for the stable accumulation of
Shuji Kaneko et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 22(1), 82-92 (2002-01-05)
The physical interaction between the presynaptic vesicle release complex and the large cytoplasmic region linking domains II and III of N-type (Ca(v)2.2) calcium channel alpha(1)B subunits is considered to be of fundamental importance for efficient neurotransmission. By PCR analysis of
Thomas A Leonard et al.
Cell, 144(1), 55-66 (2011-01-11)
Protein kinase C (PKC) isozymes are the paradigmatic effectors of lipid signaling. PKCs translocate to cell membranes and are allosterically activated upon binding of the lipid diacylglycerol to their C1A and C1B domains. The crystal structure of full-length protein kinase
Scott E Jarvis et al.
The Journal of biological chemistry, 277(46), 44399-44407 (2002-09-11)
We have previously reported that syntaxin 1A, a component of the presynaptic SNARE complex, directly modulates N-type calcium channel gating in addition to promoting tonic G-protein inhibition of the channels, whereas syntaxin 1B affects channel gating but does not support

相關內容

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

根據您的需求選擇不同類型的蛋白酶抑制劑,防止蛋白質在分離和表徵過程中降解,保障樣品預備過程中蛋白質的安全。

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