推薦產品
產品名稱
Protease Inhibitor Cocktail, for use in purification of Histidine-tagged proteins, DMSO solution
生物源
synthetic
品質等級
形狀
DMSO solution
儲存溫度
−20°C
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一般說明
蛋白酶抑制剂的DMSO溶液专用于纯化组氨酸标签蛋白。
溶液中混有多种抑制剂成分,可特异性抑制丝氨酸、半胱氨酸、天冬氨酸、嗜热菌蛋白酶样蛋白酶和氨基肽酶。
溶液中混有多种抑制剂成分,可特异性抑制丝氨酸、半胱氨酸、天冬氨酸、嗜热菌蛋白酶样蛋白酶和氨基肽酶。
特異性
抑制丝氨酸,半胱氨酸,天冬氨酸,和嗜热菌蛋白酶样蛋白酶和氨基肽酶。
應用
混合液经过专门的优化和验证,适合分离组氨酸标签(His-tag)蛋白质,特意去除螯合剂后尤其适合用于固定化金属亲和层析(IMAC)分离。
推荐用量:1 mL溶液抑制20g大肠杆菌细胞或10g杆状病毒感染草地夜蛾蛹卵巢细胞得到的100ml细胞裂解液中的蛋白酶活性。
推荐用量:1 mL溶液抑制20g大肠杆菌细胞或10g杆状病毒感染草地夜蛾蛹卵巢细胞得到的100ml细胞裂解液中的蛋白酶活性。
特點和優勢
专门为组氨酸标签蛋白纯化而配制。
靶向多种蛋白酶,具有全面的蛋白酶抑制活性。
不加螯合剂,适合IMAC应用。
方便的包装选择。
靶向多种蛋白酶,具有全面的蛋白酶抑制活性。
不加螯合剂,适合IMAC应用。
方便的包装选择。
成分
AEBSF
乌苯美司
E-64
Pepstatin A
磷阿米酮
乌苯美司
E-64
Pepstatin A
磷阿米酮
數量
推荐采用1mL抑制从20gEscherichia coli或10g杆状病毒感染 Spodoptera frugiperda蛹卵巢细胞提取的总体积100ml的蛋白酶。
该蛋白酶抑制剂混合物已针对组蛋白标记蛋白进行了优化和测试
该蛋白酶抑制剂混合物已针对组蛋白标记蛋白进行了优化和测试
準備報告
该产品在DMSO中是澄清溶液。 推荐1mL溶液可以抑制20gE. Coli细胞或10g细胞或10g 杆状病毒感染细胞得到的100ml细胞裂解液中的蛋白酶活性。
相關產品
產品號碼
描述
訂價
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
185.0 °F - closed cup
閃點(°C)
85 °C - closed cup
從最近期的版本中選擇一個:
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相關內容
Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.
根據您的需求選擇不同類型的蛋白酶抑制劑,防止蛋白質在分離和表徵過程中降解,保障樣品預備過程中蛋白質的安全。
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